MICRO-PARA Flashcards
S. epidermidis differentiated from S. aureus
growth on MSA
S. aureus ferments mannitol while
S. epidermidis does not ferments mannitol
gram pos cocci in clusters that grows on BA from medium to large with golden yellow colonies and beta-hemolytic colonies
S. aureus
A coagulase positvie
S. aureus
What agar thay differentiate strep from staph
Catalase test
What test measures bound coagulase
Slide coagulase
It is responsible for converting fibrinogen to insoluble fibrin in measuring free coagulase
Staphylothrombin
What differentiate S. epidermidis from S. saprophyticus
S. saprophyticus resistant to Novobiocin
pinpoint, translucent gray colonies
large and deep zone of beta hemolysis on BA
Streptococci pyogenes
Orgasnism identification for Streptococci pyogenes and their positive reaction
Bacitracin Disc Test - sensitive
PYRase Test - Pyrrolidonylarlamidase - Pink or cherry red color
Direct antigent test - clumping
Virulence factor For Streptocci Pyogenes that is responsible for resistant of phagocytosis and intracellular killing
M protein
Virulence factor of S. pyogenes that mediates adherence to host cells
Lipoteichoic acid
Virulence factor in S. pyogenes that responsible for the rash in scarlet fever
Pyrogenic exotoxins
common causes of S. pyogenes
Streptococcal sore throat
Commonly cause damage to the mitral valve
nodules caused by rheumatic fever
Poststreptococcal infxn can be seen in Rheumatic fever
What test that differentiate S. pyogenes from other strep
(-) CAMP test
bullseye appearing colony
small and diffused zone of beta hemolysis
Streptococcus agalactiae
(-) Bacitracin
(-) TMP SXT
(+) CAMP Test
S. agalactiae
(+) Hippurate Hydrolysis = deep purple color
S. agalactiae
A nonhemolytic organism that grows in the presence of bile and hydrolyse esculin to esculetin, can withstand a higher salt conc. than other gram (+) cocci
enterococcus
e.bovis, e. faecalis
translucent, gray, rough margin with umbonate center
Viridans Streptococci
Commonly associated with dental caries
Streptococcus bovis = type of viridans.
basta dental - viridans - S. bovis
gram (+) diplococci
capsule
alpha hemolysis
—> Translucent colonies w/c resembles water droplet
S. pneumoniae
(+) Bactiracin Test
(-) TMP SXT
(-) CAMP
S. pyogenes
Test that identifies S. pneumoniae
Optochin Inhibition test
Bile solubility test
capsular swelling test
What org that has the ability of bacterial cells to lyse in the presence of bile salt
S. pneumoniae
What test is performed when bacteria is put in an antiserum, capsule can be seen to swell.
other name?
Capsualr swelling test
Neufeld Quellung reaction
gram (-) diplococci
kidney-bean shaped cocci
Neisseria gonorrhoeae
culture medium of choice for Neisseria gonorrhoea
Thayer-Martin medium
What is added in the Thayer-Martin Medium to:
- inhibit gram + org
- inhibit gram - org
- inhibit swarming proteus
- inhibit fungus
- vancomycin
- Cholesitn
- Trimethoprim
- Lecithin
What color is produced when an enzyme added with tetramethyl paraphenylenediamine dihydrochloride?
What is that enzyme
What is the organism where we perform ni
purple and black color colonies
Cytochrome oxidase
Neisseria gonorrhoeaa
Term when gonorrhea passes in infants through an infected birth canal
Ophthalmia neonatorum
found in small amounts in nasopharynx, then it travels into the bloodstream then to the brain, causing Meningococcus
Neisseria meningitis
ocauses fever, headache, stiff neck, increase level of PMNs in spinal fluuid
N. meningitis
What is called when N. meningitidis found in the blood stream that has abrupt onset of spiking fever, chills, asthralgias and muscle pains
Meningococcemia
T/F. N. gonorrhoeae is easily destroyed by heat or outside temperature so it must inoculate iimediately into the medium or use transport packs
true
What is the confirmatory test performed in Neisseria in order to differentiate the two?
What are its results?
Carbohydrate Fermentation Test
N. gonorrhoeae ferments glucose only
N. meningitidis ferments Glucose and Maltose
“Bamboo rod” appearance
Bacillus anthracis
spore-forming gram + bacilli that is positive for Lecithinase test
Bacillus cereus
meningitis esp. in children
Haemophilus influenzae
whooping cough
bordetella
What haemophilus is referred when it ferments glucose, sucrose and fructose
Haemophilus parainfluenzae
What haemophilus os reffered when it does not ferments any carbohydrate
Haemophilus ducreyi
An organism that has mousy or bleach-like odor in CA
H. influenzae
A term referred when nodes tend to become matted together forming an oval mass
bubo
Term used that is commonly occurs in untreated px, the skin will break down leading to sinus formation and discharge pus. this can be seen to px that has an ulcerative genital
Suppuration
“school of fish” appearance in gram-stained smear
Haemophilus ducreyi
Px with Bordetella pertussis, together with whooping cough, a px can also have __
Subconjunctival hemorrhage
T/F. Spx used in diagnosing Bordetella pertussis is nasopharynx. Org can only be seen during paroxysmal and convalescent stage
false. catarrhal and paroxysmal
Mercury Drop colonies
Bordetella pertussis
Medium used in Bordetella pertussis when Mercury drop colonies is seen
Bordet-gengou medium
What vaccine is used to prevent Pertussis toxin
Acellular vaccine
DPT vaccine
Transport medium used when inoculation of spx at the px’s bedside is not evident. This px experienced subjunctival hemorrhage and whooping cough w/c is the most evident
Regan-Lowe transport medium
Identify Virulence factor of Bordetella pertussis
1. causes vasoconstriction and ischemic necrosis
2. facilitates attachment of bacteria to ciliated epithelial cells
3. decreased chemotaxis and phagocytosis of bacteria
4. promotes attachment of bacteria to host cells
5. Inhibits all cilia movement and regeneration of damaged cells
6. lymphocytosis, sensitixation of histamine, activation of insulin production that leads to hypoglycemia
- Dermonecrotic toxin
- Filamentous hemagglutinin
- Adenylate cyclase oxin
- Agglutinogens
- Tracheal toxin
- Pertussis toxin
What is the pigment that responsible for the pink colonies of Serratia marcescens?
prodigiosin
colored colonies with greenish metallic sheen on EMB
E. coli
large mucoid colonies
Klebsiella penumoniae
fish-eye colonies
Enterobacter aerogenes/cloacae
Jet black colonies on bismuth sulfite agar
Salmonella typhi
Primarily a pediatric disease with most infxn in children 6 mos - 10 yrs . this also referred as Bacillary dysentery
Shigella
Gram neg coccobacilli with variable motility
fermwents lactose and other sugars with the production of acid and gas
index of fecal contamination
E. coli
Common cause of acute gastroenteritis associated with consumption of improperly cooked seafood
Vibrio parahaemolyticus
responsible for rapidly progressive wound infxn after exposure to contaminated seawater and septicemia after consumption of raw oyster
Vibrio vulnificus
Primary habitat of Helicobacter pylori
Hman gastric mucosa
