MICRO-PARA Flashcards

1
Q

S. epidermidis differentiated from S. aureus

A

growth on MSA

S. aureus ferments mannitol while
S. epidermidis does not ferments mannitol

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2
Q

gram pos cocci in clusters that grows on BA from medium to large with golden yellow colonies and beta-hemolytic colonies

A

S. aureus

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3
Q

A coagulase positvie

A

S. aureus

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4
Q

What agar thay differentiate strep from staph

A

Catalase test

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5
Q

What test measures bound coagulase

A

Slide coagulase

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6
Q

It is responsible for converting fibrinogen to insoluble fibrin in measuring free coagulase

A

Staphylothrombin

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7
Q

What differentiate S. epidermidis from S. saprophyticus

A

S. saprophyticus resistant to Novobiocin

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8
Q

pinpoint, translucent gray colonies
large and deep zone of beta hemolysis on BA

A

Streptococci pyogenes

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9
Q

Orgasnism identification for Streptococci pyogenes and their positive reaction

A

Bacitracin Disc Test - sensitive
PYRase Test - Pyrrolidonylarlamidase - Pink or cherry red color
Direct antigent test - clumping

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10
Q

Virulence factor For Streptocci Pyogenes that is responsible for resistant of phagocytosis and intracellular killing

A

M protein

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11
Q

Virulence factor of S. pyogenes that mediates adherence to host cells

A

Lipoteichoic acid

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12
Q

Virulence factor in S. pyogenes that responsible for the rash in scarlet fever

A

Pyrogenic exotoxins

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13
Q

common causes of S. pyogenes

A

Streptococcal sore throat

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14
Q

Commonly cause damage to the mitral valve
nodules caused by rheumatic fever

A

Poststreptococcal infxn can be seen in Rheumatic fever

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15
Q

What test that differentiate S. pyogenes from other strep

A

(-) CAMP test

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16
Q

bullseye appearing colony
small and diffused zone of beta hemolysis

A

Streptococcus agalactiae

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17
Q

(-) Bacitracin
(-) TMP SXT
(+) CAMP Test

A

S. agalactiae

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18
Q

(+) Hippurate Hydrolysis = deep purple color

A

S. agalactiae

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19
Q

A nonhemolytic organism that grows in the presence of bile and hydrolyse esculin to esculetin, can withstand a higher salt conc. than other gram (+) cocci

A

enterococcus

e.bovis, e. faecalis

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20
Q

translucent, gray, rough margin with umbonate center

A

Viridans Streptococci

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21
Q

Commonly associated with dental caries

A

Streptococcus bovis = type of viridans.

basta dental - viridans - S. bovis

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22
Q

gram (+) diplococci
capsule
alpha hemolysis
—> Translucent colonies w/c resembles water droplet

A

S. pneumoniae

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23
Q

(+) Bactiracin Test
(-) TMP SXT
(-) CAMP

A

S. pyogenes

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24
Q

Test that identifies S. pneumoniae

A

Optochin Inhibition test
Bile solubility test
capsular swelling test

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25
Q

What org that has the ability of bacterial cells to lyse in the presence of bile salt

A

S. pneumoniae

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26
Q

What test is performed when bacteria is put in an antiserum, capsule can be seen to swell.

other name?

A

Capsualr swelling test

Neufeld Quellung reaction

27
Q

gram (-) diplococci
kidney-bean shaped cocci

A

Neisseria gonorrhoeae

28
Q

culture medium of choice for Neisseria gonorrhoea

A

Thayer-Martin medium

29
Q

What is added in the Thayer-Martin Medium to:

  1. inhibit gram + org
  2. inhibit gram - org
  3. inhibit swarming proteus
  4. inhibit fungus
A
  1. vancomycin
  2. Cholesitn
  3. Trimethoprim
  4. Lecithin
30
Q

What color is produced when an enzyme added with tetramethyl paraphenylenediamine dihydrochloride?

What is that enzyme

What is the organism where we perform ni

A

purple and black color colonies

Cytochrome oxidase

Neisseria gonorrhoeaa

31
Q

Term when gonorrhea passes in infants through an infected birth canal

A

Ophthalmia neonatorum

32
Q

found in small amounts in nasopharynx, then it travels into the bloodstream then to the brain, causing Meningococcus

A

Neisseria meningitis

33
Q

ocauses fever, headache, stiff neck, increase level of PMNs in spinal fluuid

A

N. meningitis

34
Q

What is called when N. meningitidis found in the blood stream that has abrupt onset of spiking fever, chills, asthralgias and muscle pains

A

Meningococcemia

35
Q

T/F. N. gonorrhoeae is easily destroyed by heat or outside temperature so it must inoculate iimediately into the medium or use transport packs

A

true

36
Q

What is the confirmatory test performed in Neisseria in order to differentiate the two?

What are its results?

A

Carbohydrate Fermentation Test

N. gonorrhoeae ferments glucose only

N. meningitidis ferments Glucose and Maltose

37
Q

“Bamboo rod” appearance

A

Bacillus anthracis

38
Q

spore-forming gram + bacilli that is positive for Lecithinase test

A

Bacillus cereus

39
Q

meningitis esp. in children

A

Haemophilus influenzae

40
Q

whooping cough

A

bordetella

41
Q

What haemophilus is referred when it ferments glucose, sucrose and fructose

A

Haemophilus parainfluenzae

42
Q

What haemophilus os reffered when it does not ferments any carbohydrate

A

Haemophilus ducreyi

43
Q

An organism that has mousy or bleach-like odor in CA

A

H. influenzae

44
Q

A term referred when nodes tend to become matted together forming an oval mass

A

bubo

45
Q

Term used that is commonly occurs in untreated px, the skin will break down leading to sinus formation and discharge pus. this can be seen to px that has an ulcerative genital

A

Suppuration

46
Q

“school of fish” appearance in gram-stained smear

A

Haemophilus ducreyi

47
Q

Px with Bordetella pertussis, together with whooping cough, a px can also have __

A

Subconjunctival hemorrhage

48
Q

T/F. Spx used in diagnosing Bordetella pertussis is nasopharynx. Org can only be seen during paroxysmal and convalescent stage

A

false. catarrhal and paroxysmal

49
Q

Mercury Drop colonies

A

Bordetella pertussis

50
Q

Medium used in Bordetella pertussis when Mercury drop colonies is seen

A

Bordet-gengou medium

51
Q

What vaccine is used to prevent Pertussis toxin

A

Acellular vaccine
DPT vaccine

52
Q

Transport medium used when inoculation of spx at the px’s bedside is not evident. This px experienced subjunctival hemorrhage and whooping cough w/c is the most evident

A

Regan-Lowe transport medium

53
Q

Identify Virulence factor of Bordetella pertussis
1. causes vasoconstriction and ischemic necrosis
2. facilitates attachment of bacteria to ciliated epithelial cells
3. decreased chemotaxis and phagocytosis of bacteria
4. promotes attachment of bacteria to host cells
5. Inhibits all cilia movement and regeneration of damaged cells
6. lymphocytosis, sensitixation of histamine, activation of insulin production that leads to hypoglycemia

A
  1. Dermonecrotic toxin
  2. Filamentous hemagglutinin
  3. Adenylate cyclase oxin
  4. Agglutinogens
  5. Tracheal toxin
  6. Pertussis toxin
54
Q

What is the pigment that responsible for the pink colonies of Serratia marcescens?

A

prodigiosin

55
Q

colored colonies with greenish metallic sheen on EMB

A

E. coli

56
Q

large mucoid colonies

A

Klebsiella penumoniae

57
Q

fish-eye colonies

A

Enterobacter aerogenes/cloacae

58
Q

Jet black colonies on bismuth sulfite agar

A

Salmonella typhi

59
Q

Primarily a pediatric disease with most infxn in children 6 mos - 10 yrs . this also referred as Bacillary dysentery

A

Shigella

60
Q

Gram neg coccobacilli with variable motility
fermwents lactose and other sugars with the production of acid and gas
index of fecal contamination

A

E. coli

61
Q

Common cause of acute gastroenteritis associated with consumption of improperly cooked seafood

A

Vibrio parahaemolyticus

62
Q

responsible for rapidly progressive wound infxn after exposure to contaminated seawater and septicemia after consumption of raw oyster

A

Vibrio vulnificus

63
Q

Primary habitat of Helicobacter pylori

A

Hman gastric mucosa