Micro Midterm Lab Practical Flashcards
Where do you dispose... Used glass test tubes Used glass slides Broken glass Used latex gloves Paper towels used in bench disinfection Uncontaminated trash Contaminated Petri plates Qtip swabs used to clean microscopes
Discard racks Disinfectant basin Cardboard broken glass receptacle Autoclave trash Regular trash Regular trash Autoclave trash Regular trash
Function of ocular
The eye piece that Magnifies an image ten times
Function of objective lenses
Magnify an image at varying amounts
Function Of Condenser
Concentrates the light beam onto the specimen
Function of the course focus knob
Refine resolution of images on a larger scale
Function of iris diaphragm lever
Enlarges the iris diaphragm to let increase light intensity
Function of mechanical stage knobs
Allows you to move the specimen around to find things
Function oF Condenser height adjustment knobs
Adjust. Height of condenser
Function of condenser centering screws
Centers the condenser
Function of diopter ring
Allows you to focus the individual eyepiece To your eye.
Function of base diaphragm
H
Function of fine focus knob
Adjust the resolution at a smaller scale
Function of stage clip
Hold slide. In. Place
Describe the steps to cleaning. A microscope properly
- Rotate nosepiece to 5xs objective
- Remove excess. Oil from oil immersion objective with qtip
- Clean all lenses with lens cleaner and qtips and dry
- Turn down rheostat and turn off light switch
- Replace cover
Explain. Purpose of using immersion oil who using 100x objective
It minimizes the amount of light that is refracted or lost and therefore. Increases resolution of the image.
Name two major types of microscopes that have been developed and. And name the difference between. These two types. Of microscopes
Compound microscope- uses light
Electron microscopes- uses electrons to produce. Image
List 4 examples. Of compound microscopes and star the example you will use in lab
Phase contrast
*bright field
Dark
Fluorescent
Define magnification
Ability of. Microscope to enlarge an object
Name two magnifying lenses. In a compound microscope
Objective lens
Ocular/eyepiece lens
List. Common names. And the magnification of the four objectives you will. Use in this. Lab
Scanning. Objective- 4x
Lower power objective- 10x
High power. Objective- 40
Oil immersion objective-100x
How is total magnification of an object calculated?
Ocular x objective = total magnification
State the. Two. Factors that. Determine. How we’ll an object. Can e seen with a. Microscope
Resolution and magnification
Define. Resolution
Describes. How clear specimen will appear when viewed through the lenses
Name the. Two components that influence the resolving. Power of. A microscope
Numerical aperture and. Wavelength of light