Micro And Nanosensors Flashcards

1
Q

What is a biosensor

A

Measure chemical information (metabolites, cytokines…)

Can convert this information into electrical signals

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2
Q

How are different signals generated and transduced?

A

Electrical:
Electrons ➡️ redox➡️ measurement eg. Diabetic glucose sensor
Ions➡️ activity and conductivity
Optical:
Photons➡️ fluorescence, scattering
Mechanical:
Mass change➡️ mechanical measurement of electrical changes

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3
Q

How can biosensors be used to detect antibodies

A
Nano cantilevers
Ligands immobilised on nano levers
Antibiotic binds and distorts lever
There is a deflection
Produces signal
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4
Q

How do nanowires work

A

When there is binding to wire

Change in current and voltage of nanowire

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5
Q

How have nano sensors been used in next generation sequencing

A

Amplify DNA
Beads will carry copy of single sequence
Beads added to microplate wells
Record the pH change in each well when 4dNTPs added sequentially
When base incorporated get voltage change

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6
Q

What is single molecule DNA sequencing

A

DNA polymerase immobilised on waveguide

Fluorescence from each base will be detected

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7
Q

Whats another example of DNA sequencing using nano sensors

A

Nanopore

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8
Q

Other examples of nanobiosensors

A

Quantum dots - fluorescent biosensor

PEBBLES - fluoresce when glucose is present. Measure glucose levels

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