Biomaterials For Vascular Regeneration Flashcards
What are the ideal qualities for artificial vasculature
Have an endothelial layer Have smooth muscle cells Good elasticism Suture retention, easy to handle Can survive high pressure Biocompatibility Low production costs Easy to store Readily available
What kind of cells can be used for vascular repair
Stem cells:
Embryonic SC
Bone marrow SC
Reprogramming body cells to be pluripotent
What are the different types of vascular scaffolds
Natural scaffold:
Directly seeded onto cells
Can be stored
Reduced mechanical properties with lower burst pressure
Synthetic scaffolds: Extensive availability Affordable Can get acidic by products Size mismatch leads to graft failure
Decellularised scaffold: No adverse immunological reactions Natural architecture of tissues More expensive If not completely decellularised then immune reaction Salvage in event of complication is low
What materials are used to enhance cell attachment or survival
Naturally occurring materials:
Collagen, fibrin
Biocompatible but poor mechanical properties
Non degradable synthetic polymers
Good availability
Biodegradable synthetic polymers
Can get inflammation from degradation of products
Loss of mechanical properties after degradation
How do cell sheets work?
Cells allowed to grow on a surface which is thermosensitive
At room temperature the cell sheet detaches from the polymer and comes off as a sheet that can be used
Can roll them up to make vessel
How else can we make vessels from cell sheets?
Make two sheets from different cell types and roll one on top of the other
