Micro 5 (Test 1) Flashcards
The gram stain is…
differential stain
The acid fast stain is to identify the class of bacteria called _____
mycobacteria
In order to identify a capsule, spore, or flagella, which stain is used?
specialized stain
Methylene Blue Stain is used in:
Simple Stain
Crystal Violet Stain is used in:
Gram Stain
Safranin Stain is used in:
Acid Fast Stain
Janus green is used in:
Spore Stain
The preparation of a _____ is the process is which bacteria is transferred to a glass slide.
smear
An _____ is always used to transfer bacteria from one media to another.
inoculating loop
The process of _____ must be performed every time the loop becomes contaminated to ensure sterilization and prevent cross contamination.
flaming the loop
TRUE or FALSE: If a liquid medium is given, a drop of water on a slide is needed in the transfer process.
FALSE
TRUE or FALSE:
When using the inoculation loop, bacterial residue must be visible to the naked eye to ensure that bacteria were actually transferred.
FALSE
TRUE or FALSE:
When emulsifying the water and the bacteria, if the water turns turbid or murky then too much bacteria has been transferred.
TRUE
The loop should be placed under the flame until it turns…
red hot
Heat fixing should be done for _____ seconds
2 (no more no less)
Wash the slide for _____ seconds and pat dry with _____ paper.
30 seconds, biblous
TRUE or FALSE:
The key to staining is to transfer as much bacteria as possible in order to see them under the microscope.
FALSE
Gram positive organisms stain the color _____ because they accept the dye _____.
blue, crystal violet
Gram negative organisms stain the color _____ because they accept the dye _____.
red, saffranin
The purpose of certain dyes in the Gram stain: Mordant/ _____
iodine
The purpose of certain dyes in the Gram stain: decolorizer/_____
Ethyl Alchohol
The purpose of certain dyes in the gram stain: counter stain/_____
safranin
Purpose of the certain dyes in the Gram stain: primary stain/_____
crystal violet
The difference between G (+) and Gram (-) bacteria is the construction and chemistry of their _____
cell wall
More susceptible to antibiotics +/-
+
More complex nutritional requirements +/-
+
Relatively thin cell wall +/-
-
Have a thick, single celled wall +/-
+
Posses more lipids in their cell walls +/-
-
Higher presence of the chemical hexoseamine
+
Cannot posses a spore +/-
-
More susceptible to inhibition by dyes +/-
+
The only G (-) cocci are called
Neisseria
The crystal violet - iodine complex is tightly held by gram
+
After alcohol treatment, G (-) cells are nearly invisible unless they are counterstained with a second dye (true or false)
TRUE
- Identify the reproductive pattern in which bacteria appear scattered with no pattern
Micro
- Identify the reproductive pattern in which bacteria appear in groups of four
Tetra
- Identify the reproductive pattern in which bacteria appear in clumps like grapes
Staphlo
- Identify the reproductive pattern in which bacteria in groups of two (Cells remain linked in pairs)
Diplo
- Identify the reproductive pattern in which bacteria appear in groups of 8
Sarcina (spelling)
Cells remain linked in long chains like a necklaces
Strepto
Iceberg like bodies floating through the cell:
Inclusion bodies/metachromatic
A chemical found in the cell wall of bacteria:
A chemical found in the cell wall of bacteria: gylcocalyx
