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Microbiology > Micro 5 (Test 1) > Flashcards

Micro 5 (Test 1) Flashcards

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Biology 101 flashcards
1
Q

The gram stain is…

A

differential stain

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2
Q

The acid fast stain is to identify the class of bacteria called _____

A

mycobacteria

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3
Q

In order to identify a capsule, spore, or flagella, which stain is used?

A

specialized stain

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4
Q

Methylene Blue Stain is used in:

A

Simple Stain

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5
Q

Crystal Violet Stain is used in:

A

Gram Stain

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6
Q

Safranin Stain is used in:

A

Acid Fast Stain

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7
Q

Janus green is used in:

A

Spore Stain

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8
Q

The preparation of a _____ is the process is which bacteria is transferred to a glass slide.

A

smear

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9
Q

An _____ is always used to transfer bacteria from one media to another.

A

inoculating loop

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10
Q

The process of _____ must be performed every time the loop becomes contaminated to ensure sterilization and prevent cross contamination.

A

flaming the loop

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11
Q

TRUE or FALSE: If a liquid medium is given, a drop of water on a slide is needed in the transfer process.

A

FALSE

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12
Q

TRUE or FALSE:
When using the inoculation loop, bacterial residue must be visible to the naked eye to ensure that bacteria were actually transferred.

A

FALSE

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13
Q

TRUE or FALSE:
When emulsifying the water and the bacteria, if the water turns turbid or murky then too much bacteria has been transferred.

A

TRUE

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14
Q

The loop should be placed under the flame until it turns…

A

red hot

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15
Q

Heat fixing should be done for _____ seconds

A

2 (no more no less)

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16
Q

Wash the slide for _____ seconds and pat dry with _____ paper.

A

30 seconds, biblous

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17
Q

TRUE or FALSE:

The key to staining is to transfer as much bacteria as possible in order to see them under the microscope.

A

FALSE

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18
Q

Gram positive organisms stain the color _____ because they accept the dye _____.

A

blue, crystal violet

19
Q

Gram negative organisms stain the color _____ because they accept the dye _____.

A

red, saffranin

20
Q

The purpose of certain dyes in the Gram stain: Mordant/ _____

A

iodine

21
Q

The purpose of certain dyes in the Gram stain: decolorizer/_____

A

Ethyl Alchohol

22
Q

The purpose of certain dyes in the gram stain: counter stain/_____

A

safranin

23
Q

Purpose of the certain dyes in the Gram stain: primary stain/_____

A

crystal violet

24
Q

The difference between G (+) and Gram (-) bacteria is the construction and chemistry of their _____

A

cell wall

25
Q

More susceptible to antibiotics +/-

A

+

26
Q

More complex nutritional requirements +/-

A

+

27
Q

Relatively thin cell wall +/-

A

-

28
Q

Have a thick, single celled wall +/-

A

+

29
Q

Posses more lipids in their cell walls +/-

A

-

30
Q

Higher presence of the chemical hexoseamine

A

+

31
Q

Cannot posses a spore +/-

A

-

32
Q

More susceptible to inhibition by dyes +/-

A

+

33
Q

The only G (-) cocci are called

A

Neisseria

34
Q

The crystal violet - iodine complex is tightly held by gram

A

+

35
Q

After alcohol treatment, G (-) cells are nearly invisible unless they are counterstained with a second dye (true or false)

A

TRUE

36
Q
  1. Identify the reproductive pattern in which bacteria appear scattered with no pattern
A

Micro

37
Q
  1. Identify the reproductive pattern in which bacteria appear in groups of four
A

Tetra

38
Q
  1. Identify the reproductive pattern in which bacteria appear in clumps like grapes
A

Staphlo

39
Q
  1. Identify the reproductive pattern in which bacteria in groups of two (Cells remain linked in pairs)
A

Diplo

40
Q
  1. Identify the reproductive pattern in which bacteria appear in groups of 8
A

Sarcina (spelling)

41
Q

Cells remain linked in long chains like a necklaces

A

Strepto

42
Q

Iceberg like bodies floating through the cell:

A

Inclusion bodies/metachromatic

43
Q

A chemical found in the cell wall of bacteria:

A

A chemical found in the cell wall of bacteria: gylcocalyx

Microbiology (19 decks)

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