Micro 5 (Test 1)

Question 1

The gram stain is…

Answer 1

differential stain

Question 2

The acid fast stain is to identify the class of bacteria called _____

Answer 2

mycobacteria

Question 3

In order to identify a capsule, spore, or flagella, which stain is used?

Answer 3

specialized stain

Question 4

Methylene Blue Stain is used in:

Answer 4

Simple Stain

Question 5

Crystal Violet Stain is used in:

Answer 5

Gram Stain

Question 6

Safranin Stain is used in:

Answer 6

Acid Fast Stain

Question 7

Janus green is used in:

Answer 7

Spore Stain

Question 8

The preparation of a _____ is the process is which bacteria is transferred to a glass slide.

Answer 8

smear

Question 9

An _____ is always used to transfer bacteria from one media to another.

Answer 9

inoculating loop

Question 10

The process of _____ must be performed every time the loop becomes contaminated to ensure sterilization and prevent cross contamination.

Answer 10

flaming the loop

Question 11

TRUE or FALSE: If a liquid medium is given, a drop of water on a slide is needed in the transfer process.

Answer 11

FALSE

Question 12

TRUE or FALSE:
When using the inoculation loop, bacterial residue must be visible to the naked eye to ensure that bacteria were actually transferred.

Answer 12

FALSE

Question 13

TRUE or FALSE:
When emulsifying the water and the bacteria, if the water turns turbid or murky then too much bacteria has been transferred.

Answer 13

TRUE

Question 14

The loop should be placed under the flame until it turns…

Answer 14

red hot

Question 15

Heat fixing should be done for _____ seconds

Answer 15

2 (no more no less)

Question 16

Wash the slide for _____ seconds and pat dry with _____ paper.

Answer 16

30 seconds, biblous

Question 17

TRUE or FALSE:

The key to staining is to transfer as much bacteria as possible in order to see them under the microscope.

Answer 17

FALSE

Question 18

Gram positive organisms stain the color _____ because they accept the dye _____.

Answer 18

blue, crystal violet

Question 19

Gram negative organisms stain the color _____ because they accept the dye _____.

Answer 19

red, saffranin

Question 20

The purpose of certain dyes in the Gram stain: Mordant/ _____

Answer 20

iodine

Question 21

The purpose of certain dyes in the Gram stain: decolorizer/_____

Answer 21

Ethyl Alchohol

Question 22

The purpose of certain dyes in the gram stain: counter stain/_____

Answer 22

safranin

Question 23

Purpose of the certain dyes in the Gram stain: primary stain/_____

Answer 23

crystal violet

Question 24

The difference between G (+) and Gram (-) bacteria is the construction and chemistry of their _____

Answer 24

cell wall

Question 25

More susceptible to antibiotics +/-

Answer 25

+

Question 26

More complex nutritional requirements +/-

Answer 26

+

Question 27

Relatively thin cell wall +/-

Answer 27

-

Question 28

Have a thick, single celled wall +/-

Answer 28

+

Question 29

Posses more lipids in their cell walls +/-

Answer 29

-

Question 30

Higher presence of the chemical hexoseamine

Answer 30

+

Question 31

Cannot posses a spore +/-

Answer 31

-

Question 32

More susceptible to inhibition by dyes +/-

Answer 32

+

Question 33

The only G (-) cocci are called

Answer 33

Neisseria

Question 34

The crystal violet - iodine complex is tightly held by gram

Answer 34

+

Question 35

After alcohol treatment, G (-) cells are nearly invisible unless they are counterstained with a second dye (true or false)

Answer 35

TRUE

Question 36

1. Identify the reproductive pattern in which bacteria appear scattered with no pattern

Answer 36

Micro

Question 37

2. Identify the reproductive pattern in which bacteria appear in groups of four

Answer 37

Tetra

Question 38

3. Identify the reproductive pattern in which bacteria appear in clumps like grapes

Answer 38

Staphlo

Question 39

4. Identify the reproductive pattern in which bacteria in groups of two (Cells remain linked in pairs)

Answer 39

Diplo

Question 40

5. Identify the reproductive pattern in which bacteria appear in groups of 8

Answer 40

Sarcina (spelling)

Question 41

Cells remain linked in long chains like a necklaces

Answer 41

Strepto

Question 42

Iceberg like bodies floating through the cell:

Answer 42

Inclusion bodies/metachromatic

Question 43

A chemical found in the cell wall of bacteria:

Answer 43

A chemical found in the cell wall of bacteria: gylcocalyx