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Microbiology > Micro 2 > Flashcards

Micro 2 Flashcards

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Biology 101 flashcards
0
Q

Grams iodine is considered a

A

Mordant

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1
Q

The 4 reagents used to perform a gram stain

A

Crystal violet
Grams iodine
Decolorizer
Safranin

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2
Q

What is a mordant

A

Helps cause a chemical reaction between stain and bacteria. Helps it stick

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3
Q

Decolorizer could be 2 things

A

Acetone or alcohol

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4
Q

Safranin is considered

A

Carbol fuschin

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5
Q

Why do gram pos and gram neg stain differently

A

Bc of the amt of peptidoglycan in the cell wall

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6
Q

Peptidoglycan consists of

A

Sugar and amino acids

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7
Q

What bacteria has high affinity for violet stain and stains purple

A

Gram pos

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8
Q

Gram pos stains ____ gram neg stains _____

A

Pos- purple

Neg- pink or red

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9
Q

Gram pos vs gram neg

A

Gram pos- thicker cell wall, thicker layer of peptidoglycan.

Gram neg- thinner ^. Outer lipopolysaccharide layer (LPS)

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10
Q

Outer lipopolysaccharide layer makes gram neg resistant to

A

Abx

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11
Q

3 basic forms of bacteria

A

Coccus, bacillus, spirillium

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12
Q

Staphylococci vs streptococci

A

Staph- grape like cluster

Strept- chain like

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13
Q

Sarcina

A

Cube shape- 8 stacked

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14
Q

Crystal violet precipitate on epithelial cell may be confused with

A

Gram pos cocci

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15
Q

If cells are all purple

A

May be under decolorized

16
Q

If cells are all pink

A

May be over decolorized

17
Q

How you know it’s stained properly

A

Pink nuclei and cytoplasm

18
Q

Normal psittacine stool (mute) consist of

A

95% gram pos rods and cocci. 5% gram neg rods

19
Q

Is yeast normal in birds

A

Occasionally

20
Q

Acid fast stain is a differential stain that distinguishes organisms with

A

Waxy cell walls (mycolic acid)

21
Q

Acid fast stain usually resist

A

Decolorization with acid alcohol

22
Q

Mycobacterium paratuberculosis

A

Affects cattle, johne’s dz

23
Q

Mycobacterium bovis

A

Affects cattle- TB. Zoonotic

24
Q

Organisms that stain with acid fast don’t stain well with other stains- why?

A

Resists the decolorization

25
Q

Cryptosporidium is hidden where and causes what

A

Feces. Causes diarrhea

26
Q

Ziehl neelson method aka

A

Acid fast stain

27
Q

DMSO is used to look for

A

cryptosporidium

28
Q

Acid fast picks up

A

Red/ pink

29
Q

Not acid fast stains

A

Purple

30
Q

Can you determine bacteria species from gram stain or acid fast staining alone?

A

No

Microbiology (11 decks)

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