MICRO Flashcards
what is the single most important etiologic agent of severe diarrheal dz of infants & children world wide?
rotavirus
what kind of genome does the rotavirus have?
nonenveloped, segmented dsRNA
Which virus is the major cause of food-borne, epidemic acute gastroenteritis in older children & adults?
norovirus
What is rotateq?
vaccine for rotavirus with 5 bovine-human reassortant viruses
what is rotarix?
vaccine for rotavirus with single attenuated human virus
humans are the only known reservoir for what virus that is known to cause symptoms via direct virus-mediated cell damage and tissue necrosis?
enteroviruses
where do enteroviruses initialy replicate?
in lymphoid tissue of upper respiratory tract and the gut
which type of virus causes pleurodynia (epidemic mylagia)?
group B coxsackie viruses (aka devil’s grip)
what virus causes hand-foot-mouth dz?
coxsackie virus A16
which virus causes myocarditis?
group B coxsackie virus
which virus can cause acute hemorrhagic conjunctivitis that is highly contagious?
enterovirus 70 & coxsackie A24
what kind of genome do the noroviruses have?
non-enveloped, nonsegmented, +RNA virus w/ 5’VPG & 3’ Poly A tail
which type of virus typically can be traced back to a single source like shellfish, cake frosting or salads?
norovirus
which virus has a shorter incubation period norovirus or rotavirus?
norovirus (1 day, 6-10 hrs)
Rotavirus (1-4 days)
what kind of genome does picornaviridaee have?
nonsegmented, ss +RNA
an infection by what kind of microbe can cause Guillan-Barre syndrome?
campylobacter infection
Name the E. coli serotype O157:H7
enterohemorhagic E. coli (EHEC)
what is the most common cause of traveler’s diarrhea?
ETEC
which bacteria is the leading cause of infantile diarrhea in developing countries?
EPEC
what is the most common cause of hemorrhagic colitis in the US?
EHEC
which microbe makes currant jelly mucoid colonies?
klebsiella pneumoniae
which microbe is the MCC of shigellosis in US?
S. Sonnei
what kinds of pts get shigellosis from S. Sonnei?
mostly children
Shiegella enter what kind of cells in the gut?
microfold (M) cells
name the microbe: contaminate wounds, bacteremia + UTI in hospital pts, found in feces of healthy pts
enterobacter
name the microbe: brick red opportunistic colonies causing pneumonia; found in feces of healthy pts
serratia marcescens
what are the swarming bacteria?
proteus-providencia morganella (nosocomial agents causing UTIs and antibody related diarrhea)
how does the shiga toxin work?
phage encoded cytotoxin (AB) that cleaves 28S RNA of ribosome
name the 2 microbes that produce a high amount of shiga toxin
Shigella dysenteriae & Enterohemorrhagic E. Coli
which microbe causes typhoid fever?
Salmonella Typhi
which microbe is a gram negative coccobacilli that looks like a safety pin?
yersinia entercolitica
Salmonella can be acquired from what household pets?
pet turtles
what is the name of the Virulence factor made by uropathogenic E. coli that binds to galactose disaccharides on uroepithelium
P fimbria (Pyelonephritis assoc. pili-PAP, binds P blood group antigen)
All the enteric pathogens are motile except which 3?
Enteroinvasive E. coli
Shigella
Klebsiella
which type of E. Coli is non-motile, doesn’t decarboxylate lysine and doesn’t ferment lactose?
EIEC
the invasion phenotype of EIEC is encoded by what?
high molecular weight plasmid (can be detected by PCR)
persistent diarrhea >10-14 days is likely from what?
parasite
what microbe is the #1 cause of UTIs?
E. coli
which type of e. coli can’t use sorbitol?
EHEC O157:H7 (grows white on sorbitol macConkey agar, while other e. coli are red/pink)
antibiotics are contraindicated for which type of E. coli?
EHEC (risk of HUS)
name the only species of salmonella
Salmonella enterica
describe the presentation of salmonellosis?
symptoms begin 20-72 hrs post consumption of improperly cooked food
-N/V, followed by abd. cramps & diarrhea lasting 3-4 days
what type of capsule antigen inhibits neutrophil uptake in typhoid fever?
Vi
describe the presentation of typhoid fever
insidious, rising fever, w/ headache, abd. pain
- slow pulse, mental confusion
- diarrhea late if at all
- death possible by ruptured spleen or intestine
name the microbe that doesn’t cause gastroenteritis but does likely cause a disseminated infection via the fecal-oral route
Salmonella Typhi
Name the microbe:
Gram neg., non-motile rod
Lac neg., glu+, no gas, no H2S
leukocytes prominent in stool samples
shigella
which microbe causes pseudoappendicitis?
yersinia enterocolitica (abd. pains that can mimic appendicitis)
which microbe can characteristically cause aspiration pneumonia in alcoholics?
Klebsiella pneumoniae
name the microbe: slender, curved Gram neg. rods that are motile with polar flagella, microaerophilic, make VacA, use Type III Secretion system, and Cag protein
H. pylori
what does VacA do in H. pylori?
vacuolating cytotoxin (punches holes in organelles of cells)
what does Cag in H. Pylori infections do?
rearanges cytoskeleton (modifies proteins–>cell death)
what is the most common bacterial GI infection in developed nations?
campylobacter jejuni
what are some of the most common sources of campylobacter jejuni infections?
undercooked poultry & unpasteurized milk, contaminated water
name the microbe: thin, spiral shaped Gram neg. rod with gull-winged appearance & is also microaerophilic
campylobacter jejuni
which kind of GI infection is the most common antecedent to guillain-barre syndrome?
campylobacter jejuni infection
describe the clinical syndrome of campylobacter jejuni infection
incubation of 1 wk, fever, appendicitis-like lower abd. pain
-watery diarrhea that progresses to dysentery with blood & pus
which microbe is commonly associated with poor sanitation and related to spread by contaminated water and food. Has 2-3 day incubation period, abrupt onset of vomiting & high volume watery diarrhea
V. cholerae
which microbe causes rice-water stools?
v. cholerae
what is the TCP virulence factor of V. Cholerae?
PAI-encoded pilus that allows for attachment
how does the cholera toxin work?
ADP-ribosylation of GTP-binding protein, activation of adenyl cyclase leading to Cl- secretion and high volume watery diarrhea
if you wanted to do a stool culture for v. cholerae what kinds of medium would you use?
Thiosulfate citrate Bile salts sucrose (TCBS), or MacConkey
what is the most common food borne illness in japan?
vibrio parahaemolyticus (seafood associated food poisoning)