MICR 202: exercise 1

Question 1

voltage regulator

Answer 1

red dial that controls light intensity. should be kept at mid-position.

Question 2

condenser

Answer 2

collects light from source and directs it to object on stage. should be kept at highest position.

Question 3

diaphragm

Answer 3

regulates amount of light reaching object on the stage.

Question 4

stage

Answer 4

where you put microscope slides.

Question 5

objective lenses

Answer 5

determines magnification

Question 6

ocular lenses

Answer 6

located at top of microscope. magnification of 10x.

Question 7

focusing knobs

Answer 7

large knob-course adjustment knob.

small knob-fine adjustment knob

Question 8

field of view

Answer 8

actual size of area being observed. as magnification increases, field of view decreases.

Question 9

working distance

Answer 9

distance between objective lens and object being observed.

Question 10

magnification

Answer 10

total magnification=objective lens x ocular lens (10x)

Question 11

resolving power

Answer 11

clarity of an image depends on this. it is the ability to distinguish fine detail

Question 12

how to achieve maximum resolving power

Answer 12

lenses must be clean.
condenser must be at highest position
diaphragm should be open
immersion oil must be used

Question 13

how to focus under low power

Answer 13

raise the condenser
lock in 10x lens
raise stage to highest position
focus on fine adjustment

Question 14

how to focus under oil

Answer 14

focus under 10x
rotate halfway to 100x
put drop of oil on slide
lock in 100x lens
open diaphragm
focus using fine adjustment only

Question 15

storing microscope

Answer 15

turn off light
unplug scope
remove slide
center slide holder
lower stage
push oculars together
remove all oil
lock in 10x lens