MG Deck 1 Flashcards
Describe the structure of DNA
Double-stranded helix of nucleotides that run Antiparallel
Why is the replication of DNA considered semi-conservative
Each strand of the original molecule serves as a template. New DNA contains one conserved parental strand and one new one.
Major players of DNA replication
Helicase, Topoisomerase, Primase, Ligase and DNA Polymerase
Primase
RNA Primer-start site for the enzyme that makes DNA
Helicase
The enzyme that unzips the DNA
Topoisomerase
The enzyme that relaxes the strand for helicase to do its job
Ligase
Binds (glues) the lagging strand
DNA Polymerase
Reads DNA from 3-5 prime and synthesizes (makes) DNA from 5-3 prime.
Why do DNA strands run Antiparallel?
due to space constraints
Has linear DNA and multiple origins of replication
Eukaryotes
1 origin bubble of replication
Prokaryotes
All genetic information in a cell
Genome
Molecule of DNA plus proteins
Chromosome
Who does translation?
Ribosomes
Segment of DNA encoding a functional product -has a non coding region
Gene
The coding region of an organism
Genotype
Only found in Prokaryotes
Plasmids
Substitution
The wrong base is matched.-Frameshift
Insertion
An extra base or bases are added in
Deletion
A base is removed.
Point Mutation
One nucleotide is changed.
premature stop codon
Nonsense mutation
only effects genotype-protein is not effected
Silent Mutation
Amino acid changed may or may not affect function
Missense Mutation