Methods Of Studing Cells (Microscopes) Flashcards
Define magnification
Tells you how many times bigger the image produced by the microscope is that the real life object
Define resolution
The ability to distinguish between two objects that are close together
How to convert millimetres to micrometres (mm - um)
x1000
How to convert micro meters into nanometres
x1000
Magnification equation
Magnification = Size of image / Size of real object
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A M
What optical microscopes use to form an image
Light
What is the maximum resolution of a light microscope
0.2 micrometers (um)
What is the maximum magnification of an optical microscope
x 1500
What organelles can’t be seen with an optical microscope
Ribosomes, endoplasmic reticulum, lysosomes
What do electron microscope is used for an image
Electrons
Which has higher resolution TEM or SEM
TEM
Which has greater magnification electron or light microscope
Electron
What does the images that electron microscopes produce look like
Black and white
(Can be coloured by computer) 
What are the two types of electron microscope
Transmission electron microscope’s (TEMs)
Scanning electron microscope’s (SEMs)
How does a transmission electron microscope work
Use electromagnets to focus a beam of electrons which is transmitted through the specimen
Advantages of TEMs
High resolution
Can see internal structures
Disadvantages of TEMs
Thin specimens only
Can’t view living organisms - uses vacuum so no water
No coloured image