Methods Of Cell Study Flashcards
Simple Microscope
Contains a single pair of lenses. This could only magnify what could be seen by the naked eye. Provided an enlarged image.
Zachaus Janssen
1950, a Dutch lens maker that developed the first “simple microscope”.
Robert Hooke
1665, an English scientist that developed the first compound light microscope. Could see what could not be seen with naked eye (cells). Coined the term “cell” while viewing cork.
Compound light microscope
Contains two sets of lenses that improve resolution (clarity of image).
Anton Van Leeuwenhoek
A Dutch lens maker who in 1670 developed a powerful compound light microscope that could magnify 270x.
Leeuwenhoek discovered…..
Bacteria, Protozoa, red blood cells, yeast cells and sperm.
Electron Microscope
Developed in the 1930’s. Used as high energy beam of electrons to create a magnified image seen on a CRT (cathode ray tube). Focusing is achieved with the use of powerful electromagnets.
Advantages of the Electron Microscope….
Incredible magnification over 1,000,000x. Greatly improved resolution.
Disadvantages of the Electron Microscope…..
Specimens must be specially prepared and treated with a heavy metal stain to be viewed. Living tissue can not be viewed.
The advancement of the electron microscope lead to the discovery of…….
The virus
Bi-Ocular Dissecting Microscope
Contains two oculars to provide a 3-D image of an object. Low magnification for viewing gross anatomical structure.
Phase Contrast Microscope
Provides a 3-D black an white image of a stained specimen. It can be used to view living cells.
Ultra-Centrifuge
A high speed rotating device that can be used to separate materials according to their density (molecular weight).
Micro-Dissecting Tools
Ultra fine instruments to be used under the microscope.
Microtome
A machine that is used to cut very thin sections of tissue and/or cells for microscope study.
