Methods in Immunology Flashcards
1
Q
What is a complement fixation test?
A
- Used to detect presence of antibody or antigen in sera
2
Q
What is ELISA?
A
- Enzyme-linked immunosorbent assay
- Plate based assay to detect and qualify solubles (peptides, proteins, antibodies, hormones
- Uses antibody conjugated to enzyme
- Horseradish Peroxidase (substrate TMB)
3
Q
How is an ELISA carried out?
A
4
Q
What is IGRA?
A
- Interferon gamma release assay
- Whole blood tests that can aid in diagnosis of tuberculosis
- Incubate blood with TB antigens, if person has TB they will have TB specific T cells
5
Q
What is flow cytometry?
A
- Provides rapid multiparameter analysis of single cells in solution (NIH)
- Provides size, shape, complexity, fluorescence
- Uses forward scatter and side scatter fluorescence
- Enumberates and sorts cells
- 3 major components - fluidics, optics, electronics
6
Q
What type of particles can be analyzed?
A
- Viruses, exosomes, bacteria, rbc, animal cells, plant cells, oocytes
- 100 um to 100 nm
(IMAGE 10)
7
Q
What type of data can be gathered from flow cytometry?
A
- Histogram, Dot plot, Pseudocolor, Contour
8
Q
What is forward scatter? What is side scatter?
A
FSC
- Indicates cell size
- Proportional to cell size
- Higher FCS is larger cell
SSC
- Indicates cells internal complexity/granularity
- More granules and organelles a cell has the higher the SSC
9
Q
How are FSC and SSC plotted on a graph?
A
10
Q
What is fluorescence?
A
- Tag cells using fluorescent antibodies to measure cell concentrations
- Measures single or multiple targets
11
Q
What is Gating?
A
- Selecting of data points for analysis using graphical or numerical boundaries
12
Q
How can cells be sorted in flow cytometry?
A
- Fluorescence activated cell sorting
