Methods in FITC

Question 1

How many polypeptide chains make up an antibody?

Answer 1

4

Question 2

How are the polypeptide chains of an antibody connected?

Answer 2

The polypeptide chains of an antibody are connected by disulfide bonds.

Question 3

What is the composition of the polypeptide chains in an antibody

Answer 3

An antibody consists of two heavy chains (H) and two light chains (L).

Question 4

What are the two regions found in an antibody?

Answer 4

The two regions in an antibody are the antigen-binding fragments (Fab) and the crystallizable fragment (Fc)

Question 5

What is the function of the Fab region in an antibody?

Answer 5

The Fab region of an antibody is responsible for binding to antigens. It contains the variable (V) domains or paratopes.

Question 6

What is the function of the Fc region in an antibody?

Answer 6

The Fc region of an antibody is responsible for various effector functions, such as binding to immune cells and activating complement. It contains the constant (C) domain.

Question 7

What is the approximate molecular weight of an antibody?

Answer 7

An antibody typically has a molecular weight of around 150 kilodaltons (kDa).

Question 8

What is the size of an antibody in terms of nanometers (nm)?

Answer 8

10 nm

Question 9

: How many antibody isotypes are determined by the Fc region?

Answer 9

The Fc region determines the five antibody isotypes: IgG, IgE, IgD, IgM, and IgA.

Question 10

What is the paratope?
A) Part of an antigen recognized by an antibody
B) Antigen-binding site on an antibody
C) Region on the surface of an antibody
D) Epitope-binding region on an antigen

Answer 10

b.

Question 11

Where is the paratope located?
A) Fc region of the antibody
B) Variable domain of the light chain
C) Constant domain of the heavy chain
D) Fab region of the antibody

Answer 11

B

Question 12

What is an epitope?
A) Part of an antigen recognized by an antibody
B) Antigen-binding site on an antibody
C) Region on the surface of an antibody
D) Paratope-binding region on an antigen

Answer 12

a.

Question 13

How does the paratope contribute to the immune response?
A) It facilitates neutralization of antigens
B) It serves as a constant region of the antibody
C) It activates complement proteins
D) It regulates antibody production in B cells

Answer 13

a.

Question 14

Can different antibodies recognize the same epitope?
A) Yes, as long as they have the same isotype
B) No, each antibody recognizes a unique epitope
C) Yes, if they have the appropriate paratope structure
D) No, each antibody recognizes a different epitope

Answer 14

c.

Question 15

Q1: What is a monoclonal antibody?
A) An antibody produced by a single B cell clone
B) An antibody that recognizes multiple epitopes on an antigen
C) An antibody produced by combining different antibody types
D) An antibody that is only found in certain tissues or organs

Answer 15

a.

Question 16

How are monoclonal antibodies generated?
A) By isolating antibodies from multiple donors
B) By mixing different types of antibodies together
C) By cloning a single B cell producing a specific antibody
D) By exposing B cells to a diverse range of antigens

Answer 16

c.

Question 17

What is a polyclonal antibody?
A) An antibody produced by a single B cell clone
B) An antibody that recognizes multiple epitopes on an antigen
C) An antibody produced by combining different antibody types
D) An antibody that is only found in certain tissues or organs

Answer 17

b.

Question 18

How are polyclonal antibodies generated?
A) By isolating antibodies from multiple donors
B) By mixing different types of antibodies together
C) By cloning a single B cell producing a specific antibody
D) By exposing B cells to a diverse range of antigens

Answer 18

a.

Question 19

Which type of antibody is more commonly used in immunohistochemistry (IHC) staining?
A) Monoclonal antibodies
B) Polyclonal antibodies
C) Both monoclonal and polyclonal antibodies
D) Neither monoclonal nor polyclonal antibodies

Answer 19

b

Question 20

Which type of antibody is typically used in therapeutic applications?
A) Monoclonal antibodies
B) Polyclonal antibodies
C) Both monoclonal and polyclonal antibodies
D) Neither monoclonal nor polyclonal antibodies

Answer 20

a.

Question 21

What is the advantage of polyclonal antibodies in research and diagnostics?
A) They provide a broader immune response against pathogens
B) They allow for the detection of multiple targets simultaneously
C) They are readily available and cost-effective to produce
D) They offer a greater specificity and uniformity

Answer 21

a

Question 22

What is the advantage of monoclonal antibodies in research and diagnostics?
A) They provide a broader immune response against pathogens
B) They allow for the detection of multiple targets simultaneously
C) They are readily available and cost-effective to produce
D) They offer a greater variety of isotypes and subclasses

Answer 22

b

Question 23

Q6: Which of the following is true about polyclonal antibodies?
A) They exhibit a high degree of specificity and uniformity
B) They are more cost-effective to produce compared to monoclonal antibodies
C) They recognize a single epitope on an antigen
D) They are produced by a single B cell clone

Answer 23

b.

Question 24

Q1: What is the purpose of perfusion in tissue fixation?
A) To distribute fixatives throughout the brain tissue
B) To visualize cellular components in tissues
C) To preserve the structure of the vascular system
D) To enhance the fluorescence in immunohistochemistry

Answer 24

A

Question 25

Q2: What is the main advantage of perfusion fixation?
A) It allows for the preservation of tissue structure and cellular components
B) It enhances the fluorescence signal in immunohistochemistry
C) It facilitates the preparation of specific protocols for PFA
D) It is a cost-effective method for tissue fixation

Answer 25

A

Question 26

Which system is utilized for perfusion fixation in brain tissue?
A) Respiratory system
B) Digestive system
C) Vascular system
D) Nervous system

Answer 26

C.