Gene k.o. Flashcards
Which approach starts with a known gene and explores its function and phenotypic consequences?
a) Forward genetics
b) Reverse genetics
c) Comparative genomics
d) Epigenetics
b
How are transgenic mice typically generated?
a) By introducing foreign DNA sequences into the mouse genome
b) Through spontaneous mutations in the mouse genome
c) By selectively breeding mice with desired traits
d) Using viral vectors to deliver genes into mouse brain cells
a
What is the term used for the introduction of a single gene or DNA sequence into an organism’s genome?
a) Synthetic constructs
b) Simple transgenes
c) Point mutants
d) Knock-outs
b
what are forward genetics based on?
a. From phenotype to genotype
b. from genotype to phenotype
a.
reverse genetics are based on…
a. phenotype to genotype
b. genotype to phenotype
b
Knock-in…
a targeted introduction of a transgene into a silent genomic area
b. introduction of a new gene into organism
c. targeted introduction of mutations into an existing gene
c.
transgenic means…
a targeted introduction of a transgene into a silent genomic area
b. introduction of a new gene into organism
c. targeted introduction of mutations into an existing gene
b
Why do we inject into the male pronucleus?
a. because of its size
b. because of its location
c. because of sexism
a
Whats a marker of embryonic stem cells?
a. PAX6
b. LIF5
c. Oct4
d. KCC
c
Choose correct order
a. introduce gene into ES cells, select cells containing gene, inject transformed ES cells back into blastocyst, transport into surrogate, breed
b. breed, transport into surrogate, introduce gene into ES cells, select cells containing gene, inject transformed ES cells back into blastocyst
c. introduce gene into ES cells, select cells containing gene, inject transformed ES cells back into blastocyst, breed, transport into surrogate
a.
construction of a classical targeting vector by…
a. Homologous recombination
b. Positive selection
c. Negative selection
d. all of the above
d
Select correct statement:
a. Positive selection marker: most often neomycin phosphotransferase; Negative selection: ganciclovir
b. Pos. selection: ganciclovir; neg. selection: neo casette
a.
Establishing targeted ESCs…
a. is achieved by Southern blot and PCR
b. is achieved by Nothern blot and PCR
c. is achieved by western blot and PCR
a.
Knock-down experiments involve:
a) Completely eliminating gene function
b) Selectively inactivating a gene in specific tissues
c) Reducing gene expression levels temporarily or partially
d) Introducing foreign genes into an organism’s genome
c
What distinguishes conditional knockouts from general knockouts?
a) Conditional knockouts allow for the selective inactivation of a gene in specific tissues or developmental stages
b) General knockouts are reversible, while conditional knockouts are permanent
c) Conditional knockouts can only be achieved in invertebrate model organisms
d) General knockouts require external stimuli to induce gene inactivation
a
What is the Cre-lox system commonly used for?
a) Generating transgenic mice
b) Manipulating gene expression in specific tissues
c) Studying DNA replication mechanisms
d) Inducing random mutations in the genome
b
What is Cre in the Cre-lox system?
a) A DNA-binding protein
b) A viral vector
c) An enzyme that catalyzes recombination
d) A gene regulatory element
c) An
How does the Cre-lox system work?
a) Cre protein binds to lox sites, resulting in DNA recombination
b) Lox sites bind to specific genes, altering their expression
c) Cre protein induces mutations in targeted genes
d) Lox sites act as transcriptional enhancers for specific genes
a)
In the Cre-lox system, what is a loxP site?
a) A specific DNA sequence recognized by Cre protein
b) A gene encoding Cre protein
c) A promoter region for Cre gene expression
d) A modified DNA segment used for gene knockouts
a)
What is the purpose of the FRT and Flp system?
a) Generating transgenic mice
b) Manipulating gene expression in specific tissues
c) Studying DNA replication mechanisms
d) Creating site-specific DNA recombination
d)
