Cryo- EM

Question 1

Primary purpose of X-ray crystallography?

A. Visualizing cellular organelles in their native context
B. Comparing multiple sequences of biological molecules
C. Determining the three-dimensional atomic structure of molecules
D. Predicting protein structures using deep learning algorithms

Answer 1

c

Question 2

Which technique has shown impressive performance in predicting protein structures?

A. Cryo-electron tomography
B. X-ray crystallography
C. Multiple sequence alignment
D. ALPHAFOLD2

Answer 2

d.

Question 3

What is the primary advantage of cryo-electron microscopy?

A. Provides high-resolution 3D structural information
B. Allows determination of the three-dimensional atomic structure
C. Enables alignment and comparison of multiple sequences
D. Predicts protein structures using deep learning algorithms

Answer 3

a.

Question 4

What distinguishes cryo-electron tomography from traditional cryo-electron microscopy?

A. Captures 2D images from different angles
B. Allows visualization of cellular organelles
C. Involves freezing the sample in a thin layer of vitrified ice
D. Reconstructs 3D tomograms from tilted 2D images

Answer 4

d.

Question 5

What is the primary challenge associated with the phase problem in X-ray crystallography?

A. Determining the crystal lattice parameters
B. Measuring the intensity of diffracted X-rays
C. Identifying the unit cell of the crystal
D. Obtaining the phases of diffracted X-rays

Answer 5

d.