Methods Flashcards
Samples that may be used for hexokinase method
Heparin, EDTA, fluoride, oxalate, citrate
Most specific glucose method
Hexokinase method
Cholesterol reference method
Abell, Levy and Brodie Method
Uses hexane extraction after hydrolysis with alcoholic KOH followed by reaction with Liebermann-Burchardt color reagent
Abell, Levy and Brodie method
TAG reference method
Modified Van Handel and Zilversmith
Involves alkaline hyrolysis, solvent extraction with chloroform and treated with silicic acid, color rxn with chromotropic acid giving rise to a pink color
Modified Van Handel and Zilversmith
HDL reference method
Ultracentrifugtion, precipitation with heparin MnCl2 and Abell-Kendall assay (3-step method)
LDL research method
Beta quantification method