METHOD OF STAINING Flashcards by Queen Alcaraz | Brainscape

Q

most widely used stain

A

Lactophenol Cotton (aniline) Blue (LPCB)

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Q

preserve structures; slides can be made permanent

A

lactic acid and glycerol

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Q

dislodges fungal structures

A

Lactophenol Cotton (Aniline) Blue

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Q

clears specimen using 10-20% KOH

A

Potassium hydroxide (KOH)

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Q

stains chitin of fungi

A

Calcofluor white

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Q

can be mixed with KOH; detect fungi rapidly due to bright fluorescence

A

calcofluor white

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Q

detects cryptococcus in CSF

A

nigrosin/indian ink

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Q

diagnostic of meningitis

A

nigrosi/india ink

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Q

mucoplysaccharide for capsule of Cryptococcus

A

alcian blue or mucicarmine

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Q

rapid detection of P. jirovecii from lung biopsy or BAL

A

Toluidine O/Blue

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Q

examination of bone marrow and blood smear

A

wright’s stain

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Q

detects H. capsulatum and Cryptococcus spp.

A

wright’s stain

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Q

detects fungi

A

periodic acid schiff

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Q

in PAS, add ___ mins time if counterstain will

A

5

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Q

melanin in fungal cell wall

A

masson fontana

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Q

differentiates melanin from hemosiderin

A

masson fontana

Q

detects fungi in histologic sections

A

methanamine silver

Q

best stain for fungal elements

A

methenamine silver