CULTURE MEDIA

Question 1

general purpose/routine media for fungi

Answer 1

Sabouraud Dextrose Agar (SDA)

Question 2

Selective media for pathogenic fungi, which inhibits saprophytic fungi

Answer 2

SDA with cycloheximide

Question 3

fungi that feeds on the dead

Answer 3

saprobe

Question 4

primary recovery of saprobic and pathogenic fungi

Answer 4

Braine-Heart infusion Agar (BHIA), Potato flake agar, Sabouraud dextrose with brain-heart infusion (SABHI) agar

Question 5

Primary recovery of pathogenic fungi not including dermatophytes

Answer 5

brain heart infusion agar (BHIA) with antibiotics, yeast extract phosphate agar with ammonia

Question 6

antibiotics used in BHIA with antibiotics

Answer 6

chloramphenicol, cycloheximide, gentamicin

Question 7

inhibits bacteria

Answer 7

chloramphenicol, gentamicin

Question 8

inhibits saprophytic fungi

Answer 8

cycloheximide

Question 9

enhances recovery and sporulation of Blastomyces and
Histoplasma

Answer 9

yeast extract phosphate

Question 10

Primary recovery of pathogenic, cycloheximide sensitive fungi exclusive of dermatophytes

Answer 10

inhibitory mold agar

Question 11

Primary recovery of dermatophytes; recommended as screening medium

Answer 11

dermatophyte test medium

Question 12

primary recovery of dermatophytes (E.M.T species)

Answer 12

mycosel/mycobiotic agar

Question 13

Detection of ascospores in ascosporogenous yeasts (e.g., Saccharomyces spp.)

Answer 13

acetate ascospore agar

Question 14

• Identification of Cryptococcus, Trichosporon, and Rhodotorula spp.

Answer 14

Christensen’s urea agar

Question 15

• Separation of Trichophyton mentagrophytes from Trichophyton rubrum

Answer 15

Christensen’s urea agar

Question 16

(+)urease and hair perforation

Answer 16

Trichophyton mentagrophytes

Question 17

Differentiation of chlamydia spp by chlamydospore production

Answer 17

Cornmeal agar with Tween 80 & trypan blue

Question 18

Differentiation of Aspergillus spp

Answer 18

Czapek’s agar/ Czapek Dox Agar

Question 19

Identification of Crytococcus neoforman and Cryptococcus gatti

Answer 19

nigerseed agar/bird seed agar

Question 20

Demonstration of conidia formation and the pigment production by Trichophyton rubrum; preparation of microslide cultures & sporulation of dermatophytes

Answer 20

Potato dextrose agar

Question 21

identification of yeasts by determining fermentation

Answer 21

yeast fermentation broth

Question 22

indicator or yeasts fermentation broth

Answer 22

bromcresol purple

Question 23

Identification of yeasts by determining carbohydrate assimilation

Answer 23

Yeast nitrogen base agar

Question 24

For enhanced recovery of Malassezia furfur

Answer 24

SDA with Olive oil

Question 25

For Trichophyton mentagrophytes

Answer 25

Hair baiting in media