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MYCOLOGY > CULTURE MEDIA > Flashcards

CULTURE MEDIA Flashcards

1
Q

general purpose/routine media for fungi

A

Sabouraud Dextrose Agar (SDA)

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2
Q

Selective media for pathogenic fungi, which inhibits saprophytic fungi

A

SDA with cycloheximide

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3
Q

fungi that feeds on the dead

A

saprobe

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4
Q

primary recovery of saprobic and pathogenic fungi

A

Braine-Heart infusion Agar (BHIA), Potato flake agar, Sabouraud dextrose with brain-heart infusion (SABHI) agar

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5
Q

Primary recovery of pathogenic fungi not including dermatophytes

A

brain heart infusion agar (BHIA) with antibiotics, yeast extract phosphate agar with ammonia

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6
Q

antibiotics used in BHIA with antibiotics

A

chloramphenicol, cycloheximide, gentamicin

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7
Q

inhibits bacteria

A

chloramphenicol, gentamicin

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8
Q

inhibits saprophytic fungi

A

cycloheximide

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9
Q

enhances recovery and sporulation of Blastomyces and
Histoplasma

A

yeast extract phosphate

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10
Q

Primary recovery of pathogenic, cycloheximide sensitive fungi exclusive of dermatophytes

A

inhibitory mold agar

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11
Q

Primary recovery of dermatophytes; recommended as screening medium

A

dermatophyte test medium

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12
Q

primary recovery of dermatophytes (E.M.T species)

A

mycosel/mycobiotic agar

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13
Q

Detection of ascospores in ascosporogenous yeasts (e.g., Saccharomyces spp.)

A

acetate ascospore agar

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14
Q
  • Identification of Cryptococcus, Trichosporon, and Rhodotorula spp.
A

Christensen’s urea agar

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15
Q
  • Separation of Trichophyton mentagrophytes from Trichophyton rubrum
A

Christensen’s urea agar

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16
Q

(+)urease and hair perforation

A

Trichophyton mentagrophytes

17
Q

Differentiation of chlamydia spp by chlamydospore production

A

Cornmeal agar with Tween 80 & trypan blue

18
Q

Differentiation of Aspergillus spp

A

Czapek’s agar/ Czapek Dox Agar

19
Q

Identification of Crytococcus neoforman and Cryptococcus gatti

A

nigerseed agar/bird seed agar

20
Q

Demonstration of conidia formation and the pigment production by Trichophyton rubrum; preparation of microslide cultures & sporulation of dermatophytes

A

Potato dextrose agar

21
Q

identification of yeasts by determining fermentation

A

yeast fermentation broth

22
Q

indicator or yeasts fermentation broth

A

bromcresol purple

23
Q

Identification of yeasts by determining carbohydrate assimilation

A

Yeast nitrogen base agar

24
Q

For enhanced recovery of Malassezia furfur

A

SDA with Olive oil

25
Q

For Trichophyton mentagrophytes

A

Hair baiting in media

MYCOLOGY (10 decks)

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