Membrane bilayer & proteins (1) Flashcards
What are the dry mass weight proportions of a membrane’s composition?
- Lipid: 40%
- Protein: 60%
- Carbohydrate: 1-10%
What is the function of a membrane bilayer?
- Continuous, highly selective permeability barrier
- Controls enclosed chemical environment
- Communication
- Recognition (signalling, adhesion, immune surveillance)
- Signal generation in response to stimuli
What proportion of a membrane bilayer’s weight is water?
- 20%
Outline the structure of a phospholipid.
- 2 FA chains: may have kinks
- Glycerol molecule
- Interchangeable head
What is the glycerol and FA chains together called?
- Phosphatidyl backbone
What do the heads to the phospholipid have to be? Give some examples.
- Polar
- Choline/serine/ethanolamine/inositol.
What causes the kink in the FA chain?
- Cis double bond.
What are glycolipids?
- Sugar containing lipids base on sphingomyelin backbone
What are the different types of glycolipids?
- Cerebrosides: Head group sugar monomer
- Gangliosides: Oligosaccharides head group
What four types of phospholipid motion?
- Flexion (dynamic vibration)
- Rotation
- Lateral diffusion
- Flip-flop (rare as hydrophobic through hydrophilic environment)
What is the structure of cholesterol?
- Polar head
- Rigid planar steroid ring structure
- Non-polar hydrocarbon tail
How does cholesterol join to other phospholipid molecules?
- Via beta-OH group
What is the role of cholesterol in membranes?
- Maintains integrity to prevent crystalline islands
- Reduces phospholipid packing: increases fluidity
- Reduces phospholipid chain motion: decreases fluidity (helpful at high temps as reduces vibration so less damage)
What is the evidence for proteins in membranes?
- Facilitated diffusion
- Ion gradients
- Specificity of cell responses
- Membrane fractionation and gel electrophoresis
- Freeze fracture
What are the motions of proteins in a bilayer?
- Conformational change
- Rotational
- Lateral
- NOT flip-flop