Media Types Flashcards

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1
Q

Chocolate Agar

A

Cooked horse blood

Haemophilus and Nesseria spp.

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2
Q

CNA

A

Columbia Colistin-Nalidixic Agar

Gram Positive Bacteria

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3
Q

PEA

A

Phenylethyl Alcohol Agar

Gram Positive Cocci &

Anaerobic Gram Negative Bacilli

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4
Q

SSA

A

Streptococcal Selective Agar

Selective for S. pyogenes and S. agalatiae

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5
Q

EMB

A

Eosin Methylene Blue

Selective/Differential

Enteric Gram Negative Bacilli

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6
Q

MAC

A

MacConkey Agar

Selective/Differential

Enteric Gram Negative Bacilli

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7
Q

SMAC

A

MacConkey Sorbitol Agar

Selective for E. coli O157:H7

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8
Q

HE

A

Hektoen Enteric

Selective/Differential

Enteric Bacteria, Inhibits Normal Flora

  • Non-Pathogens are Orange-Salmon Pink
  • Non-Lactose Fermenters are Green-Blue Green
  • H2S colonies produce black precipitate
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9
Q

XLD

A

Xylose Lysine Deoxycholate

Selective/Differential

Salmonella and Shigella from stool

  1. Salmonella: red colonies with black centers
  2. Shigella: colorless or red colonies
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10
Q

SS

A

Salmonella-Shigella Agar

Selective

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11
Q

GN (Broth)

A

Gram-Negative Broth

Selective/Enrichment

Salmonella and Shigella

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12
Q

Selenite Broth

A

Enrichment for Salmonella

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13
Q

Tetrathionate Broth

A

Enrichment for Salmonella

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14
Q

Campy BA

A

Campylobacter Blood Agar

Selective/Enrichment

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15
Q

TM

A

Modified Thayer-Martin

Selective/Enrichment

  1. N. gonorrhoeae
  2. N. meningitidis
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16
Q

Martin-Lewis

A

Selective/Enrichment

  1. N. gonorrhoeae
  2. N. meningitidis
17
Q

NYC

A

New York City Media

Selective/Enrichment

  1. N. gonorrhoeae
  2. N. meningitidis
18
Q

SBA

A

Sheep Blood Agar

Most non-fastidious bacteria

19
Q

BBE

A

Bacteroides Bile-Esculin Agar

Selective/Differential

Bacteroides fragilis: Hydrolysis of esculin indicated by blackening of agar

20
Q

CDC

A

Anaerobic blood agar

Enrichment

Fastidious anaerobes

21
Q

CCFA

A

Cycloserine cefoxitine fructose egg yolk agar

Selective for Clostridium dificile

22
Q

Cooked Meat Medium

A

Isolation Clostridium

23
Q

EYA

A

Egg Yolk Agar

Lecthinase and lipase production

Clostridia and Fusibacterium

24
Q

LKV

A

Laked Kanamycin-Vancomycin blood agar

Selective

Bacteroides and Prevotella

25
Q

THIO

A

Thioglycollate Broth

Grows anything, separates aerobes from anaerobes from top to bottom of tube

26
Q

Colony characteristics

A

Shape, Appearance, Margins, Color, Size

27
Q

Cellular Characteristics

A

Gram -/+, beading/bipolar staining, spores, size, chains/clusters/ect.