Media Types Flashcards
Chocolate Agar
Cooked horse blood
Haemophilus and Nesseria spp.
CNA
Columbia Colistin-Nalidixic Agar
Gram Positive Bacteria
PEA
Phenylethyl Alcohol Agar
Gram Positive Cocci &
Anaerobic Gram Negative Bacilli
SSA
Streptococcal Selective Agar
Selective for S. pyogenes and S. agalatiae
EMB
Eosin Methylene Blue
Selective/Differential
Enteric Gram Negative Bacilli
MAC
MacConkey Agar
Selective/Differential
Enteric Gram Negative Bacilli
SMAC
MacConkey Sorbitol Agar
Selective for E. coli O157:H7
HE
Hektoen Enteric
Selective/Differential
Enteric Bacteria, Inhibits Normal Flora
- Non-Pathogens are Orange-Salmon Pink
- Non-Lactose Fermenters are Green-Blue Green
- H2S colonies produce black precipitate
XLD
Xylose Lysine Deoxycholate
Selective/Differential
Salmonella and Shigella from stool
- Salmonella: red colonies with black centers
- Shigella: colorless or red colonies
SS
Salmonella-Shigella Agar
Selective
GN (Broth)
Gram-Negative Broth
Selective/Enrichment
Salmonella and Shigella
Selenite Broth
Enrichment for Salmonella
Tetrathionate Broth
Enrichment for Salmonella
Campy BA
Campylobacter Blood Agar
Selective/Enrichment
TM
Modified Thayer-Martin
Selective/Enrichment
- N. gonorrhoeae
- N. meningitidis
Martin-Lewis
Selective/Enrichment
- N. gonorrhoeae
- N. meningitidis
NYC
New York City Media
Selective/Enrichment
- N. gonorrhoeae
- N. meningitidis
SBA
Sheep Blood Agar
Most non-fastidious bacteria
BBE
Bacteroides Bile-Esculin Agar
Selective/Differential
Bacteroides fragilis: Hydrolysis of esculin indicated by blackening of agar
CDC
Anaerobic blood agar
Enrichment
Fastidious anaerobes
CCFA
Cycloserine cefoxitine fructose egg yolk agar
Selective for Clostridium dificile
Cooked Meat Medium
Isolation Clostridium
EYA
Egg Yolk Agar
Lecthinase and lipase production
Clostridia and Fusibacterium
LKV
Laked Kanamycin-Vancomycin blood agar
Selective
Bacteroides and Prevotella
THIO
Thioglycollate Broth
Grows anything, separates aerobes from anaerobes from top to bottom of tube
Colony characteristics
Shape, Appearance, Margins, Color, Size
Cellular Characteristics
Gram -/+, beading/bipolar staining, spores, size, chains/clusters/ect.