Media For Primary Isolation

Question 0

Primary isolation and maintainance of culture

Answer 0

SDA

Question 1

Procedure for media for primary isolation

Answer 1

Cultured incubated 30*C or RT

Question 2

In SDA what is added in order to enhances growth of dermatophyte

Answer 2

Thiamine

Question 3

Isolation of pathogenic fungi from contaminated material

Answer 3

Mycosel or mycobiotic

Question 4

It is in the mycosel or mycobiotic that inhibits bacterial growth

Answer 4

Chloramphenicol

Question 5

In mycosel it inhibits growth of saprophytes

Answer 5

Cyclo heximide

Question 6

Isolation and maintainance of yeast phase of systemic fungi

Answer 6

Brain heart Infusion Agar

Question 7

What temperature is used in BHI for isolation and maintainance

Answer 7

35*C

Question 8

BHI cultures what fungi

Answer 8

Actinomyces

Question 9

Recovery of blastomyces and histoplasma

Answer 9

SABHI Agar

Question 10

What is added in the recovery of Blastomyces and Histoplasma in SABHI

Answer 10

10% sheep blood

Question 11

Conversion of Blastomyces and histoplasma to yeast phase

Answer 11

Sabouraud dextrose with brain heart infusion agar

Question 12

For identification of histoplasma , Blastomyces, coccidioides

Answer 12

Yeast Extract Agar

Question 13

Most saprophytes yeast and bacteria either do not grow or produce no color change

Answer 13

Dermatophyte Test Medium

Question 14

Stimulate Chlamydospores production for C.albicans

Answer 14

Cornmeal Agar

Question 15

It is added in corn meal agar to differentiate T.rubrum from T.mentagrophytes

Answer 15

1% glucose

Question 16

Stimulate fungal sporulation use for slide culture

Answer 16

Cornmeal agar

Question 17

Promotes optimal production of hyphae and blastospore

Answer 17

Cornmeal agar with tween 80

Question 18

Presumptive identification of Candida

Answer 18

Cornmeal agar with tween 80

Question 19

Species identification of Aspergelii

Answer 19

Czapek’s Agar

Question 20

Demonstrate ascospores production in saccharomyces

Answer 20

Ascospores agar

Question 21

In birdman’s agar what’s the color of C.neoforman

Answer 21

Brown pigment

Question 22

In Birdman’s agar Cryptococcus sp. and Candida pigment

Answer 22

Grow without pigment production

Question 23

Differentiate M.audouinii and M.canis

Answer 23

Rice agar

Question 24

M.audouinii is blank in rice agar and M.canis is blank

Answer 24

-,+

Question 25

Separate T.mentagrophytes from T.rubrum

Answer 25

Urease test medium

Question 26

Identification sp. of Trichopyton

Answer 26

Trichopyton agar