Media For Primary Isolation Flashcards
Primary isolation and maintainance of culture
SDA
Procedure for media for primary isolation
Cultured incubated 30*C or RT
In SDA what is added in order to enhances growth of dermatophyte
Thiamine
Isolation of pathogenic fungi from contaminated material
Mycosel or mycobiotic
It is in the mycosel or mycobiotic that inhibits bacterial growth
Chloramphenicol
In mycosel it inhibits growth of saprophytes
Cyclo heximide
Isolation and maintainance of yeast phase of systemic fungi
Brain heart Infusion Agar
What temperature is used in BHI for isolation and maintainance
35*C
BHI cultures what fungi
Actinomyces
Recovery of blastomyces and histoplasma
SABHI Agar
What is added in the recovery of Blastomyces and Histoplasma in SABHI
10% sheep blood
Conversion of Blastomyces and histoplasma to yeast phase
Sabouraud dextrose with brain heart infusion agar
For identification of histoplasma , Blastomyces, coccidioides
Yeast Extract Agar
Most saprophytes yeast and bacteria either do not grow or produce no color change
Dermatophyte Test Medium
Stimulate Chlamydospores production for C.albicans
Cornmeal Agar
It is added in corn meal agar to differentiate T.rubrum from T.mentagrophytes
1% glucose
Stimulate fungal sporulation use for slide culture
Cornmeal agar
Promotes optimal production of hyphae and blastospore
Cornmeal agar with tween 80
Presumptive identification of Candida
Cornmeal agar with tween 80
Species identification of Aspergelii
Czapek’s Agar
Demonstrate ascospores production in saccharomyces
Ascospores agar
In birdman’s agar what’s the color of C.neoforman
Brown pigment
In Birdman’s agar Cryptococcus sp. and Candida pigment
Grow without pigment production
Differentiate M.audouinii and M.canis
Rice agar
M.audouinii is blank in rice agar and M.canis is blank
-,+
Separate T.mentagrophytes from T.rubrum
Urease test medium
Identification sp. of Trichopyton
Trichopyton agar
