Direct Microscopic Examination Flashcards
In KOH increases rate of clearing , enables better visualization of fungal elements
Gentle heat
Rapid method for detection of fungal element in clinical material
KOH 10-20%
Preserves and stain fungi shades of blue
Lactophenol cotton blue
For identification of C.neoformans
India ink or nigrosin
Negative staining in nigrosin is to demonstrate blank in C.neoforman
Capsule
Sharp delineation of fungal elements in clinical specimen by fluorescence microscopy
Calcofluor white
Infected hair fluoresce under U.V. Light
Wood’s lamp
Color of hair in wood lamp
Bright yellow green
Differentiation of fungi in tissue scrapings
PAS
In PAS carbohydrate fungus wall stains what color
Purplish
Differentiate Nocardia from Actinomyces
AFS
Ideal for Tinea verso color
Acridine Orange
Acridine color for fungus and for epithelial cell
Green fluorescent for fungus
Orange for epithelial
Best stain for B.dermatitidis
Papanicolaou