Direct Microscopic Examination Flashcards

0
Q

In KOH increases rate of clearing , enables better visualization of fungal elements

A

Gentle heat

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1
Q

Rapid method for detection of fungal element in clinical material

A

KOH 10-20%

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2
Q

Preserves and stain fungi shades of blue

A

Lactophenol cotton blue

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3
Q

For identification of C.neoformans

A

India ink or nigrosin

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4
Q

Negative staining in nigrosin is to demonstrate blank in C.neoforman

A

Capsule

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5
Q

Sharp delineation of fungal elements in clinical specimen by fluorescence microscopy

A

Calcofluor white

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6
Q

Infected hair fluoresce under U.V. Light

A

Wood’s lamp

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7
Q

Color of hair in wood lamp

A

Bright yellow green

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8
Q

Differentiation of fungi in tissue scrapings

A

PAS

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9
Q

In PAS carbohydrate fungus wall stains what color

A

Purplish

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10
Q

Differentiate Nocardia from Actinomyces

A

AFS

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11
Q

Ideal for Tinea verso color

A

Acridine Orange

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12
Q

Acridine color for fungus and for epithelial cell

A

Green fluorescent for fungus

Orange for epithelial

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13
Q

Best stain for B.dermatitidis

A

Papanicolaou

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