MEDIA Flashcards
CSMLS Study Guide
What is the primary purpose of blood agar?
Isolation of non-fastidious microorganisms & determine hemolytic reactions.
What is the primary purpose of MacConkey agar?
Select for gram negative organisms and differentiate between lactose and non lactose fermenters.
What are the main ingredients in blood agar?
-Peptone
- Sodium chloride
- agar
- 5% Sheep blood
What are the main ingredients in MacConkey Agar?
- Crystal violet
- neutral red
- bile salts
- peptone base
- lactose
Which ingredients in MacConkey agar make it selective?
Crystal Violet & Bile salts
Which ingredients in MacConkey agar make it differential?
Neutral red & lactose
What is the primary purpose of chocolate agar?
Growth of fastidious bacteria.
What are the main ingredients in chocolate agar?
-peptone base
- sodium chloride
- Hgb
What ingredients in Chocolate agar make it enriched?
RBC are lysed when agar base is heated lysing the cells releasing X (hemin) and V (NAD) factors.
What is the primary purpose of CNA agar?
Selective for isolation of gram positive organism.
What are the main ingredients of CNA?
- Colistin
- Nalidixic acid
- 5% sheep blood
What ingredients in CNA make it selective?
Colistin and Nalidixic acid prevent growth of Gram negative organisms.
What is the primary purpose of PEA agar?
Selective for the isolation of aerobic GP, GPC and GNB organisms
What ingredients make PEA selective?
Phenylethyl alcohol inhibits GN
What are the main ingredients of PEA?
- Nutrient agar base
- phenylethyl alcohol
- 5% sheep blood
What is the primary purpose of Mannitol Agar?
Selective for Staph, differentiates between coag + staph and non pathogenic staph.
What are the main ingredients of Mannitol Agar?
- peptone base
- mannitol
- phenol red
- 7.5% NaCl
What ingredients in Mannitol Agar make it selective?
7.5% NaCl - as high salt concentration inhibits most growth except for Halotolerant organisms
What ingredients in Mannitol Agar make it differential?
Phenol red & Mannitol
What is the primary purpose of Mueller Hinton Agar?
Used for AST
What are the main ingredients in Mueller Hinton agar?
-Beef extract
- Casein
- Starch
- Agar
What is the main purpose of Brain Heart Infusion agar?
Cultivation of fastidious organisms
What are the main ingredient s of brain heat infusion media?
- Dextrose
- Pork brain
- Heart infusion
- peptone
- sodium chloride
- disodium phosphate
What is the primary purpose of Sabouraud agar?
Selective for yeast and filamentous fungi
What are the primary ingredients in Sabouraud agar?
- Myocological peptones
- dextrose
- agar
- antibiotics
What is the purpose of Loeffler medium?
Isolation and growth of Corynebacterium
What is the primary purpose of Tinsdale agar?
Selective and differentiates C.diphtheriae from other corynebacterium spp.
What is the tell all sign that a tinsdale agar has growht of C.diptheria?
Black colonies with Brown halos
What ingredient in tinsdale agar is differential?
Reduction of Potassium tellurite
What is the primary purpose of Thayer-Martin agar?
selective for N.gonorrhoeae and N.menigitidis
What are the primary ingredients in Thayer-Martin agar?
- Blood agar base
- Hgb and supplement B
What is the primary purpose of Modified Thayer Martin?
Selective for brucella spp.
what ingredient in Modified-TM differentiate it from TM agar?
- Colistin
- Nystatin
- Vanc
- Trimethoprim
What is the purpose of thioglycollate broth?
Used for the recovery of anaerobes
What are the main ingredients in thio broth?
- Pancreatic digest of casein
- soy broth
- glucose
- thioglycollate
- agar
what is the purpose of HEK agar?
Isolation of salmonella & shigella from stool
What are the main ingredients in HEK agar?
- Peptone with bile salts
- lactose
-sucrose - salicin
- ferric ammonium citrate
- bromothymol blue
- acid fuchsin
What ingredients in HEK make it selective?
Peptone base and bile salts
what ingredients in HEK make it differential?
- lactose
-sucrose - salicin
- ferric ammonium citrate
- bromothymol blue
- acid fuchsin
What is the purpose of MacConkey Sorbitol agar?
Isolation and differentiation of E.coli O157:H7
What are the main ingredients in MacConkey Sorbitol agar?
- Crystal Violet
- neutral red
- bile salts
- Peptone base
- D-sorbitol
What ingredients in SMAC make it selective?
Bile salts and crystal violet
What ingredient in SMAC make it differential?
sorbitol and neutral red
What is the purpose of Salmonella Shigella agar?
Selective for salmonella and shigella
What are the main ingredients in Salmonella-Shigella agar?
- Peptone base w Lactose
- ferric ammonium citrate
- sodium thiosulfate
- neutral red
- brilliant green
-bile salts
What ingredients in Salmonella-shigella agar make it selective?
Brilliant green and bile salts inhibit coliforms
What ingredients in Salmonella-shigella agar make it differential?
Ferric ammonium citrate, sodium thiosulfate, neutral red
What is the purpose of selenite broth?
Isolation of low numbers of salmonella
What is the purpose of XLD media?
Selective and differential for Salmonella and shigella
What are the main ingredients in XLD agar?
- Yeast extract
- lysine
- xylose
- lactose
- sucrose
- bile salts
- ferric ammonium citrate
- sodium deoxycholate
- phenol red
What ingredients in XLD media make it selective?
Bile salts,
What ingredients in XLD media make it differential?
- Lysine, xylose, lactose, sucrose
- ferric ammonium citrate
- sodium deoxycholate
- phenol red
What is the purpose of Campylobacter agar?
Isolation of Campylobacter species.
What are the main ingredients in campy agar?
- peptones
- yeast extract
- Burcella agar base
-Vancomycin - Trimethoprim
- ## Polymyxin B / colistin
What is an additional ingredient found in CCDA and Karmali campy agar?
Charcoal to neutralize toxic oxygen radicals and metabolic byproducts.
What is the purpose of CIN plate?
selective for Yersinia, and my be used for the isolation of Aeromonas.
What are the main ingredients in CIN plate?
- Peptone with Yeast
- Mannitol
- Bile salts
- Neutral red
- crystal violet
- cefsulodin, irgasan and novobiocin
What colonial morphology will yersinia have on CIN agar?
Bullseye appearance
