Mechanoreceptors (Theme C) Flashcards

1
Q

Why are extracellular action potentials biphasic?

A

Due to the relative position of the electrode to the location of the Na + influx

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2
Q

Extracellular action potentials are different sizes. What do smaller APs represent?

A

Smaller diameter axons

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3
Q

The amplitude of an intracellular AP is all-or-nothing. What is the peak amplitude primarily determined by?

A

The sodium reversal potential

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4
Q

Conduction velocity in cockroach unmyelinated sensory axons is 5-10m/s. Why is this faster than in mammalian unmyelinated axons?

A

Cockroach axons are of larger diameter than mammalian axons

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5
Q

What information does sensory adaptation provide the cockroach with?

A

Dynamic information about the rate of deflection of the sensory spine

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6
Q

What mechanism may contribute to sensory adaptation?

A

Inactivation of voltage-gated Na+ channels in the mechanoreceptor membrane

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7
Q

What is the difference between intracellular & extracellular APs (mechanoreceptor practical)?

A

Intracellular APs are all the same size - all-or-nothing principle

Extracellular APs are different sizes - are graded

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8
Q

What are the 2 factors that influence the size of the extracellular AP?

A
  1. Axon diameter - the larger the diameter, the larger the AP
  2. Distance to the electrode - closer to the electrode, the larger the AP
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9
Q

Extracellular (biphasic) APs can be positive-going then negative-going or negative-going then positive-going. Why is this?

(Mechanoreceptor practical)

A

Depends on the location of the reference & recording electrodes

  • If recording electrode is closest to spine → trace will go negative then positive
  • If reference electrode is closest to spine → trace will go positive then negative
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10
Q

At rest, would you see any APs from the cockroach spine?

A

Yes - spontaneous APs

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11
Q

What do you record when you touch a single spine (on the cockroach leg)?

A
  • Record burst of APs, all the same size - as you are only activating 1 spine
  • i.e., the axon coming out of the mechanoreceptor doesn’t change its distance from the electrode / diameter
  • Spontaneous APs still occurring
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12
Q

What do you record when you tap the bench / blow on the cockroach leg?

A
  • Record burst of APs, different sizes - because you are activating & recording different spines
  • Because each axon will be a different distance from recording electrode / diameter
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13
Q

What process does the ‘touch & hold’ stimulus demonstrate on the cockroach leg?

A

Sensory adaptation

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14
Q

What happens in the cockroach when the leg spine moves?

A

When the spine moves → dendrite of the neurone is distorted → opening mechanically-gated ion channels in the dendrite → influx +ve charge → receptor potential generated → triggers AP

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15
Q

The amplitude of a compound AP is influenced what what factors?

A
  1. The size of the axon: larger diameter axons produce greater amplitude APs
  2. The proximity of the axon to the recording electrodes: those that lie closer produce greater APs
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16
Q

What does CAP stand for?

A

Compound Action potential

17
Q

How to calculate CV from biphasic AP

A
  1. Select clear biphasic AP
  2. Measure the time taken (ms) from peak-to-peak
  3. Measure the distance (mm) between the electrodes
  4. CV = d/t (m/s)
18
Q

Compare the rates of adaptation in response to the different spines? How much do the rates vary?

A

Some spines & their nerves adapt more quickly than others.
This provides a variety of info to the cockroach about its environment.

19
Q

Describe the change in activity (in terms of amplitude and frequency) of the sensory nerve during a press-and-hold stimulation

A

Initially, the frequency and amplitude of CAPs is high. As the neurons adapt to the stimulation, both the amplitude and frequency decreases.

20
Q

What advantage would sensory neurons with different rates of adaptation provide to the cockroach?

A

Neural adaptation, or sensory adaptation, is a change over time in the responsiveness of the sensory system to a constant stimulus. This allows the organism to “focus” on changes in their environment.

By having neurons that each adapt differently, the organism may interpret more complex environmental stimuli.

21
Q

Did different degrees of deflection affect the AP response, even though you moved the spine the same direction in each instance?

A

Generally, moving the spines 180° should produce larger responses.

When the spine is deflected 180° towards the proximal segment of the leg the edge of the socket surrounding the spine acts like a fulcrum, causing greater extension of the dendrite and hence greater activation

22
Q

Larger degrees of deflection of the cockroach spines generally produce larger AP responses. What is the potential advantage of this?

A

This helps the cockroach to sense the direction of potential dangers and mount a rapid escape response when appropriate.

It also helps the cockroach to ignore stimuli from other directions that may merely signal the spine resting against a surface.