Measuring cells and organelles Flashcards
1
Q
Name the two pieces of equipment used to calibrate a microscope
A
Stage micrometer and eyepiece graticule
2
Q
What is an eyepiece graticule?
A
Fitted into the eyepiece of a microscope. marked with a graduated line.
3
Q
What is a stage micrometer?
A
Specialised microscope slide with a finely divided scale on it.
4
Q
How long is a stage micrometer in cm?
A
1cm
5
Q
The first set of 10 divisions is equal to
A
1mm or 1000 micrometers
6
Q
The second set of 10 divisions is equal to
A
0.1mm or 100 micrometers
7
Q
Instructions on how to calibrate a microscope:
A
Calibrate each objective lens separately, work out the length of one eyepiece division.
8
Q
Field of view definition
A
Circular area visible down the microscope
9
Q
Area of a circle =
A
pi r squared
