MCH-cell structure

Question 1

How is resolution limited?

Answer 1

By the wavelength of radiation

Question 2

What is sectioning?

Answer 2

Technique used for soft tissue specimens, embedded into wax to avoid distortion

Question 3

Differential staining?

Answer 3

used to distinguish between 2 different things

Question 4

What is the order of stains applied to bacteria during the gram stain technique?

Answer 4

crystal violet 2 iodine then 2 alcohol (slide is washed with it)

Question 5

What is the result for gram positive bacteria in the gram stain test?

Answer 5

Remain crystal violet

Question 6

What is the result for gram negative bacteria in the gram stain test?

Answer 6

pink/red (saffronin)

Question 7

Cell theory?

Answer 7

1)smallest unit capable of independent life
2)basic unit of all living organisms
3)arise from other cells thru cell division
4)growth ‘instruction’ passed onto new cells

Question 8

What steps do pre-prepared slides undergo?

Answer 8

1) Formaldehyde:used to preserve specimens
2) Sectioning:specimens dehydrated with alcohol and placed into a wax mould
3)staining
4)securing sample onto microscope slide and adding a cover slip

Question 9

Staining prep?

Answer 9

sample placed on a slide and allowed to dry. Passed thru a flame to heat fix it onto the slide enables it to take up stains.

Question 10

How is data from electron microscopes presented?

Answer 10

Electron micrographs

Question 11

Convert from nm to μm to mm to m

Answer 11

Divide by 1000 each time

Question 12

ESS: describe the function of the surface cell membrane

Answer 12

Controls the exchange of materials

Question 13

ESS: describe the function of the cell wall

Answer 13

offer structural support to the cell