MCAT BIOCHEMISTRY Flashcards
phosphodiester bond
link sugar molecules in a polynucleotide
peptide bond
btwn amino acids
disulfide bond
covalent bond; thiol S-S
pyrimidine
1 ring
posttranslational modification of proteins (histone acetylation)
western blot
oligiodendrocytes
cns support
amino acids that can be phosphorylated
tyrosine (Y), serine (S), and threonine (T)
choosing an antigen for vaccine
immunogenicity and toxicity are LEAST suitable
aminoacyl transferase
transfer tRNA orig bound at A to P then to E
noncompetive plot
x-axis lines intercept
competitive plot
y-axis lines intercept
RT-PCR
transcriptional reg (mRNA)
quantitative PCR
measures levels of DNA
native PAGE
separate molecules based on: electrophoretic mobility, relying on length, conformation, and charge
gel filtration
size. large elute first
ion exchange
charge
isoelectric focusing
isoelectric point (pH)
water, as a liquid solvent, does not appear
in equilibrium constant expression
Na+/K+-ATPase pumps
Na+ and K+ against their conc; 3 Na+ out and 2 K+ in
competitive inhib on km and vmax
incr km no change on vmax
noncomp inhib on km and vmax
no change on km and decr vmax
uncompet inhib on km and vmax
decr km and vmax
mixed inhib on km and vmax
incr/decr km and decr vmax
peptide bonds formed and broken by
formed via condensation (dehydration) and broken via hydrolysis
ex of enantiomers
D and L glucose
epimers
differ at 1 chiral carbon
anomers
differ at the anomeric carbon
centromeres
hold sister chromatids together until separated in anaphase in mitosis. High GC content
nonsense
truncation
missense
codon that codes for diff aa
transcription steps
helicase/topo, RNA polymerase II binds TATA w/in promoter region
foramen ovale
connects right and left atria
ductus arteriosus
connects pulm artery to aorta
ductus venousus
connects umbilical vein to inf vena cava
law of independent assortment
9:3:3:1
operon:
- structural genes = DNA that codes for protein
- operator gene = repressor binding site
- promoter gene = RNA pol 1st binding site
transformation
from environ into genetic material (antibiotic resist)
conjugation
sex pili (male)
transduction
bacteriophage acquires genetic info from host
z/e stereochem
double bonds
keq = 1
deltaG is 0; no ATP produced/rqd
keq > 1
spontaneous