MBC HY

Question 1

Where are glycolipids found on the cell membrane?

Answer 1

Outer leaflet

Question 2

Where are SGLUT found in intestinal epithelial cells?

Answer 2

Apical side

Question 3

Where are GLUT5 found in intestinal epithelial cells?

Answer 3

Basolateral side

Question 4

What happens to solute transport in the intestine when Na+ conc. decreases in ECF?

Answer 4

Solute transport decreases, Glucose & amino acids

Question 5

What are examples of receptor & ligand Transcytosis?

Answer 5

1. Secretion of Ig into Saliva; 2. Maternal IgG secretion in to placenta

Question 6

What stage does the nuclear membrane dissolve?

Answer 6

Prometaphase (disassembly of 2 nuclear lamins)

Question 7

What is the difference b/w necrosis & apoptotsis on a Southern blot?

Answer 7

Necrosis: random cuts thus “solid lanes
Apoptosis: laddering

Question 8

What is the histochemical marker for lysosomes?

Answer 8

Acid phosphatase

Question 9

What are the steps in sorting and packaging of lysosomal proteins?

Answer 9

1. lysosomal hydrolases modified by M6P in Cis-Golgi

2. M6P receptor on TGN for transport to Golgi

Question 10

What is Tay-Sachs?

Answer 10

Lysosomal storage disorder resulting in accumulation of gangliosides

Question 11

What are examples of mitochondrial disorders (low ATP production)?

Answer 11

Myoclonic epilepsy with ragged fibers; LHON

Question 12

What are examples of peroxisomal disorders?

Answer 12

Zelwegger syndrome: defective import of peroxisomal proteins

Question 13

Where is Actin found?

Answer 13

Microvilli and stereocilia

Question 14

Where are IF found?

Answer 14

Keratin, vimentin, neurofilaments. lamins

Question 15

What is an example of a disorder associated with MT?

Answer 15

Alzheimer’s disease: Tau protein stabilize axonal MT

Question 16

Inhibitors of MT formation?

Answer 16

Colchicine; vinculin; vinblastine; vincristine; taxol

Question 17

Fx of pRb in cell cycle?

Answer 17

(Rb) active: Keep regulatory protein E2F attached
when poorly phosphorylated
(Rb) INACTIVE: hyperphosphorylated by growth
factor and signaling of MAP kinase …then
increased cyclin D-cdk4/6 activity as well as
E-cdk2 allows the cell to move into G1 by
dissociation of E2F
E2F is freed to activate gene expression of
thymideine kinase and DNA polymerase to
progess toward S phase

Question 18

Fx of p53 in cell cycle?

Answer 18

1. Regulates Cell Cycle: Stimulates transcription of Cdk inhibitory proteins (CKIs); 2. Initiation of Apoptosis via p53 if p21 stop has not allow a repair
   to be done