Materials For PCR Flashcards
1
Q
7 materials needed?
A
- Template DNA
- DNA oligonucleotide primers
- free dNTPs
- Taq ploymerase
- Mg2+
- Buffer
- High grade water
2
Q
Why is template DNA needed?
A
Primers bind to;
Contains target seq to be amplified
3
Q
Why border seq must be known?
A
Pair of oligonucleotide primers must hybridise to DNA molecule
4
Q
free dNTPs are?
A
Present in excess
->make new copies of target DNA
5
Q
Taq polymerase is?
A
Thermostable
->withstand temp charges w/o denaturation
6
Q
Mg2+ is used to?
A
Cofactor; activate Taq polymerase
