Adv. and dis. of PCR Flashcards
1
Q
What are the four adv.?
A
- amplify millions of copies of target DNA in short period of time;
- genetic screening;
- amplifying seq from minute amts of target DNA;
- robustness
2
Q
Why PCR is able to amplify millions of copies in short period of time?
A
double every cycle
3
Q
Why is amplifying seq from minute amts of target DNA an advantage?
A
samples are limiting
4
Q
Why is robustness an adv?
A
amplifying sequences that are badly degraded
5
Q
What are the four limitations?
A
- amplified seq must be known;
- >cannot be used to isolate genes that had not been studied before; - limit to the the length of the DNA seq that can be copied;
- DNA contamination may affect the result;
- not error-free
- Taq DNA polymerase lacks 3’->5’ exonuclease proof reading ability