mast cells

Question 1

mast cell stabilizers

Answer 1

zaditor

Question 2

mast cell subtypes:

Answer 2

CTMC, MMC

Question 3

mast cells circulate as immature/mature cells

Answer 3

immature; mature in peripheral tissues

Question 4

cluster of differentiation:

Answer 4

refers to cell surface markers, so it allows immunophenotyping, but surface expression of CDs are not specific for one cell or particular lineage

Question 5

Mast cell CDs

Answer 5

CD34+, CD117+, Cd38-

CD34 facilitates cell migration
CD117 receptor for tyrosine kinase type 3, binds to SCF and forms dimer that activates phosphorylation cascade, signal transduction. cell survival, proliferation, differentiation

= markers for mast cells

Question 6

GM-CSF inhibits/promotes mast cell development

Answer 6

inhibits

Question 7

IL-3 stimulated proliferation of which types of mast cells?

Answer 7

CTMC (bone marrow derived mast cells)

Question 8

IFN-g supresses/induces apoptosis of mast cells

Answer 8

induces

IFN-g suppresses SCF_mediated differentiation MCps

Question 9

TNF-a induces/suppreses MC development

Answer 9

TNF-a induces MC development

Question 10

SCF and NGF suppress/induce apoptosis

Answer 10

suppress

Question 11

Compare and contrast CTMC and MMC

Answer 11

CTMC: safranin red, fixative insensitive, T cell independent, SCF dependent; MCchymase/tryptase; lifespan 6 mo

MMC: alcian blue, sensitive to formaldehyde so use carney fixative, T cell dependent; MCtryptase; lifespan <40 d

Question 12

what location has highest mast cell concentrations?

Answer 12

dogs: pinnae and chin, superficial dermis, post-capillary venues

Cats: pinnae, paws, superficial dermis