Making DNA Fragments Flashcards
1
Q
What is recombinant DNA technology?
A
The transfer of a fragment of DNA from one organism to the other
2
Q
What are the different methods for making DNA fragments?
A
1 - Using reverse transcriptase
2 - Using restriction endonuclease enzymes
3 - Using a gene machine
3
Q
Why is it possible to transfer DNA?
A
The genetic code and transcripton and translation mechanisms are universal
4
Q
What is cDNA?
A
Complementary DNA
5
Q
What is a palindromic sequence?
A
A sequence of DNA that has complementary base pairs that read the same in opposite directions
6
Q
What are sticky ends?
A
Small tails of unpaired bases at the end of a DNA fragment
7
Q
What are sticky ends used for?
A
Binding the DNA fragment to another piece of DNA that has sticky ends with complementary sequences
