Amplifying DNA Fragments Flashcards
1
Q
What is in vivo cloning?
A
Where the gene copies are made within a living organism. As the organism grows and divides, it replicates the DNA
2
Q
What is in vitro cloning?
A
Where the gene copies are made outside of a living organism using the polymerase chain reaction (PCR)
3
Q
What is a vector?
A
Something used to transfer DNA into a cell e.g. Plasmid, Bacteriophage
4
Q
Describe the role of DNA ligase in in vivo cloning
A
Used to join the sticky ends of the DNA fragment containing the target gene and the vector DNA together
5
Q
What is a host cell?
A
The cell the recombinant DNA/ target gene is transferred to
6
Q
What is a transformed cell?
A
A vector containing the target gene
7
Q
What are marker genes used for?
A
Identifying transformed cells e.g. fluorescence
