Major Enzymes Of DNA Replication

Question 1

Unwinds DNA helix

Answer 1

Helipads

Question 2

Stabilizes single stranded DNA and prevents the two strands at the replication fork from reforming

Answer 2

Single stranded binding protein

Question 3

Avoids twisting of DNA ahead of the replication fork (to the right), by cutting DNA, turning it, and rejoining it

Answer 3

Topoisomerase

Question 4

Assembled RNA primers in the 5’ to 3’ direction to initiate a new DNA strand

Answer 4

Promase

Question 5

Main replication enzyme in E. coli; extends the RNA primers by adding DNA nucleotides to it

Answer 5

DNA polymerase III

Question 6

Escherichia coli enzyme that uses its 5’ to 3’activity to remove the RNA previously synthesized by Okazaki fragment, replaces the RNA nucleotide with DNA nucleotides

Answer 6

DNA polymerase I

Question 7

Tethers DNA polymerase III to the DNA template, making replication more efficient

Answer 7

Sliding clamp

Question 8

Seals nicks left between adjacent RNA primers replace with DNA

Answer 8

DNA Ligase