Magnification and Calibration Flashcards
What is magnification?
How many times larger the image is than the actual size of the object being viewed
What does the resolution change?
the amount of detail
What is diffraction?
The tendency fort light waves to spread as they pass close to a physical object.
What is the resolution limited by?
the diffraction of light as it passes through samples and lenses. The light waves diffract as it passes the specimen.
Why is some detail of specimen lost when viewed through a microscope?
The structures present in the specimens are very close to each other and the light reflected from individual structures can overlap due to diffraction. This means the structures are no longer seen as separate entities, and detail is lost.
What is the calculation for magnification?
Magnification= size of image/ actual size of object
How do you measure the size of a of a sample under a microscope?
With an eyepiece graticule and stage micrometer
What is an eyepiece graticule?
A glass disc marked with a fine scale of 1-100. The scale has no units and remains unchanged whichever object lens is in place. The relative size of the divisions, however, increases with each increase in magnification.
How is the scale on the graticule calibrated at each magnification?
Using a stage micrometer
What is the formula triangle for magnification?
