M7 Experiments Flashcards
Test for Peptide Bonds. A general test for Protein and Polypetides
Biuret Test
Test for the presence of @-amino acids in proteins.
Ninhydrin Test
Test for the presence of aromatic amino acids
Xanthoproteic test
Test for the presence of tryptophan
Hopkins-Cole Test
Test for phenolic compounds. also used to detect presence of tyrosin
Millon’s Test
Test for the presence of cysteine or cystine
Lead Sulfide Test
Reagent of Biuret test
NaOh + CuSO4
Reagent of ninhydrin test
2,2 dihydroxyindane- 1,3-dione
reagent of xanthoproteic test
HNO3 + NaOH
reagent of hopkin’s cole
Glyoxylic acid +H2SO4
Reagent of Millon’s test
HgNO3 in HNO3
test samples of biuret test
gelatin, egg albumin, glycine
test samples of ninhydrin test
egg albumin, alanine, gelatin
test samples of xanthoproteic test
Egg albumin, tyrosine, alanine, phenylalanine
test samples of hopkin-cole’s test
albumin, casein, tryptophan, gelatin
test samples of millon’s test
casein, tyrosine, phenol
test samples of lead sulfide test
albumin, tyrosine, casein
reagent of lead-sulfide test
NaOH + PbAc
Positive result of biuret test
Violet-pink color
negative result of biuret test
blue color
positive result of ninhydrin test
blue-violet complex
negative result of ninhydrin test
clear
proline on ninhydrin test
yellow
positive result of xanthoproteic test
yellow/oraneg
negative result of xanthoproteic test
clear
positive result of hopkins-cole test
violet ring at junction
negative result of hopkins cole test
clear
positive result of millons test
red ppte
negative result of millons test
clear
positive result of lead sulfide test
black/brown ppte
negative result of lead sulfide
clear
positive test samples of biuret test
egg albumin, gelatin
positive samples of ninhydrin test
egg albumin, gelatin, alanine
positive test samples of millons test
tyrosine, casein
positive test samples of lead sulfide
albumin
