M6 Flashcards
The function of the dideoxy (dd) nucleotides that are used in the Sanger method of DNA sequencing is to
Incorporate into newly replicated DNA strands and terminate their synthesis
The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes
Direct selection possible
The following steps must be performed to make a bacterium produce human protein
Eukaryotic transcription Reverse transcription Restriction enzyme DNA ligase Transformation Prokaryotic transcription Translation
Southern blotting is used to detect a specific
DNA.
In the large-scale production of a particular human protein in E. coli cells, the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the C-terminus. The purpose of this modification was
to facilitate purification of the expressed protein though binding to an affinity column containing chelated nickel.
What is one of the advantages of producing human growth hormone (HGH) from a cloned gene in a bacterial cell?
Larger quantities are available
The restriction enzyme EcoRI cuts between the G and the A of the sequence GAATTC. Which statement is true about this enzyme?
It leaves sticky ends of sequence AATT.
Which choice is an example of “recombinant DNA”?
a bacterial plasmid combined with a human gene
What is a clone?
an exact copy of a DNA, cell or organism
Microarray technology is a process in which:
genes active within tissues are identified using a complementary DNA probe.
A “transgenic” animal
contains a foreign gene
What is the advantage of producing human factor IX in sheep rather than in bacteria?
Sheep will produce recombinant protein that is chemically more similar to humans.
Which of the following best describes the complete sequence of steps occurring during EVERY cycle of PCR?
The mixture is heated to a high temperature to denature the double-stranded target DNA
The primers hybridize to the target DNA
DNA polymerase extends the primers to make a copy of the target DNA
In animal cloning why is an egg cell required?
Egg proteins are required for embryonic development
