M5 Flashcards
The base in the wobble position of a codon:
is the 3´ (third) base.
Which codon serves as the start codon in mRNA for translation?
AUG
Cellular protein synthesis proceeds in which direction?
amino to carboxyl terminus
What is the order of the tRNA binding sites on the 70S ribosome with respect to the 5′->3′ direction of the mRNA?
E P A
RNA polymerase:
can synthesize RNA chains de novo (without a primer)
The sigma factor of E. coli RNA polymerase:
combines with the core enzyme to confer specific binding to a promoter.
Processing of a primary mRNA transcript in a eukaryotic cell does not normally involve:
conversion of normal bases to modified bases, such as inosine and pseudouridine.
The genetic code is essentially the same for all organisms. From this, one can logically assume which of the following?
A gene from an organism can theoretically be expressed by any other organism.
Differential RNA processing may result in
the production of two distinct proteins from a single gene.
Which of these polymerases does not require a template?
Polyadenylate polymerase
In positive control of several sugar-metabolism-related operons, the catabolite activator protein (CAP) binds to DNA to stimulate transcription. What causes an increase in CAP?
decrease in glucose and increase in cAMP
The lactose operon is likely to be transcribed when:
the cyclic AMP and lactose levels are both high within the cell.
Which of the following is the first event to take place in translation in eukaryotes?
the small subunit of the ribosome recognizes and attaches to the 5’ cap of mRNA
In which direction does the RNA polymerase move?
3’ → 5’ along the template strand
The 5’- cap of eukaryotic mRNAs is a(n):
7-methylguanosine joined to the mRNA via a 5’ → 5’ triphosphate linkage.
