M3 Flashcards
Most rapid method of decalcification?
Electrolytic Method
Specimens excluded in Decalcification? (LAG BC)
Liver
Appendix
Gallbladder
Brest mass
Cervix
Thickness of Bone specimen?
1-3 mm
Remedy for EDTA?
Magnesium chloride
(T or F) Decalcification is a mandatory step.
False. It is not.
Most common method of decalcification?
Use of acid
Done by bending or pricking and probing the tissue
with needle
Physical Method or Mechanical
Mandatory step in tx processing
Dehydration
Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding tissues of bones
Decalcification
Removal of intercellular and extracellular water from tissues after fixation and prior to wax impregnation
Dehydration
A fast-acting dehydrating agent
Acetone
Acts as a double purpose as fixative and dehydrating agent?
Acetone
Alcohol
Removal of excess decalcifying agents after decalcification
Post Decalcification
Easiest method of testing for the completeness of decalcification?
Bubble Test
Used for routine dehydration
Ethyl Alcohol or Ethanol
May cause conjunctival irritation. A skin and eye irritant.
Tetrahydrofuran (THF)
Decomposes upon exposure to sunlight
Ethylene Glycol Monoethyl Ether a.k.a. Cellosolve
Ethylene Glycol Monoethyl Ether (Cellosolve) is toxix to: (BURF)
Bloody Systems
Urinary
Reproductive
Fetal
Most reliable and accurate method of testing for the completeness of decalcification?
X-ray or Radiologic Method
Double purpose of Perenyi’s Nitric Acid
Decalcifying agent
Tissue Softener
Most recommended fixative prior to decalcification, except for bone marrow?
Buffered Formalin
Optimal temperature for Nitric Acid is?
18-30 degrees Celsius
Optimal temperature for Nitric Acid?
18-30 degrees Celsius
Disadvantages of using EDTA in Decalcification?
- Inactivates alkaline phosphatase activity
- Changing of solution every 3 days (expensive)
Most rapid nitric containing acid?
Phloroglucin Nitric
A medium used in the process of infiltration?
Paraffin Wax
Acts as a double purpose as Dehydrating agent and Clearing Agent? (TDT)
Tetrahydrofuran (THF)
Diethylene dioxide (Dioxane)
Tertiary butanol / Tertiary alcohol
Also called amyl alcohol?
Pentanol
Recommended for plant and animal microtechniques?
Butyl Alcohol
Extremely slow clearing agent?
Cedarwood Oil
Routine clearing agent because it is fast acting?
Xylene
A clearing agent that is suited for urgent biopsies?
Benzene
A substitute clearing agent for Xylene & Benzene? (3 ene)
Toluene
Removal of alcohol or dehydrating agents from tissues to replaced it with a medium that will dissolve the wax with which the tissue is to be impregnated
Dealcoholization/ Clearing
T or F. Solid tissues are allowed to be air dried.
False. They are NEVER allowed to air dry.
Volume of Clearing or Dealcoholization?
10:1
Boiling point of Ethanol?
78.3°C
Boiling point of Acetone?
56°C
What is the Decalcification time of Citric Acid-Citrate Buffer Solution?
6 days
What is the pH of Citric Acid-Citrate Buffer Solution?
pH 4.5
What is the Decalcification time of Formic Acid?
2-7 days
What is the Decalcification time of Formic Acid-Sodium Citrate Solution?
3-14 days
What is the Decalcification time of Trichloroacetic Acid?
4-8 days
What is the Decalcification time of Perenyi’s Fluid?
2-7 days
What is the Decalcification time of Formol-Nitric Acid?
1-3 days
What is the Decalcification time of Aqueous Nitric Acid Solution 10%?
12 - 24 hours
What is the Decalcification time of Phloroglucin-Nitric Acid?
12-24 hours
What Clearing agent are used when the tissue is to be cleared directly from water, as in a frozen section?
Glycerin and gum syrup
T or F. Prolonged exposure to most clearing agents causes the tissue to become brittle and therefore more difficult to cut.
True
