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HISTOPATH LEC > M3 > Flashcards

M3 Flashcards

1
Q

Most rapid method of decalcification?

A

Electrolytic Method

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2
Q

Specimens excluded in Decalcification? (LAG BC)

A

Liver
Appendix
Gallbladder
Brest mass
Cervix

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3
Q

Thickness of Bone specimen?

A

1-3 mm

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4
Q

Remedy for EDTA?

A

Magnesium chloride

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5
Q

(T or F) Decalcification is a mandatory step.

A

False. It is not.

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6
Q

Most common method of decalcification?

A

Use of acid

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7
Q

Done by bending or pricking and probing the tissue
with needle

A

Physical Method or Mechanical

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8
Q

Mandatory step in tx processing

A

Dehydration

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9
Q

Removal of calcium ions or lime salts from the organic extracellular matrix, calcified collagen and surrounding tissues of bones

A

Decalcification

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10
Q

Removal of intercellular and extracellular water from tissues after fixation and prior to wax impregnation

A

Dehydration

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11
Q

A fast-acting dehydrating agent

A

Acetone

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12
Q

Acts as a double purpose as fixative and dehydrating agent?

A

Acetone
Alcohol

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13
Q

Removal of excess decalcifying agents after decalcification

A

Post Decalcification

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14
Q

Easiest method of testing for the completeness of decalcification?

A

Bubble Test

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15
Q

Used for routine dehydration

A

Ethyl Alcohol or Ethanol

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16
Q

May cause conjunctival irritation. A skin and eye irritant.

A

Tetrahydrofuran (THF)

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17
Q

Decomposes upon exposure to sunlight

A

Ethylene Glycol Monoethyl Ether a.k.a. Cellosolve

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18
Q

Ethylene Glycol Monoethyl Ether (Cellosolve) is toxix to: (BURF)

A

Bloody Systems
Urinary
Reproductive
Fetal

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19
Q

Most reliable and accurate method of testing for the completeness of decalcification?

A

X-ray or Radiologic Method

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20
Q

Double purpose of Perenyi’s Nitric Acid

A

Decalcifying agent
Tissue Softener

21
Q

Most recommended fixative prior to decalcification, except for bone marrow?

A

Buffered Formalin

22
Q

Optimal temperature for Nitric Acid is?

A

18-30 degrees Celsius

23
Q

Optimal temperature for Nitric Acid?

A

18-30 degrees Celsius

24
Q

Disadvantages of using EDTA in Decalcification?

A
  • Inactivates alkaline phosphatase activity
  • Changing of solution every 3 days (expensive)
25
Q

Most rapid nitric containing acid?

A

Phloroglucin Nitric

26
Q

A medium used in the process of infiltration?

A

Paraffin Wax

27
Q

Acts as a double purpose as Dehydrating agent and Clearing Agent? (TDT)

A

Tetrahydrofuran (THF)
Diethylene dioxide (Dioxane)
Tertiary butanol / Tertiary alcohol

28
Q

Also called amyl alcohol?

A

Pentanol

29
Q

Recommended for plant and animal microtechniques?

A

Butyl Alcohol

30
Q

Extremely slow clearing agent?

A

Cedarwood Oil

31
Q

Routine clearing agent because it is fast acting?

A

Xylene

32
Q

A clearing agent that is suited for urgent biopsies?

A

Benzene

33
Q

A substitute clearing agent for Xylene & Benzene? (3 ene)

A

Toluene

34
Q

Removal of alcohol or dehydrating agents from tissues to replaced it with a medium that will dissolve the wax with which the tissue is to be impregnated

A

Dealcoholization/ Clearing

35
Q

T or F. Solid tissues are allowed to be air dried.

A

False. They are NEVER allowed to air dry.

36
Q

Volume of Clearing or Dealcoholization?

A

10:1

37
Q

Boiling point of Ethanol?

A

78.3°C

38
Q

Boiling point of Acetone?

A

56°C

39
Q

What is the Decalcification time of Citric Acid-Citrate Buffer Solution?

A

6 days

40
Q

What is the pH of Citric Acid-Citrate Buffer Solution?

A

pH 4.5

41
Q

What is the Decalcification time of Formic Acid?

A

2-7 days

42
Q

What is the Decalcification time of Formic Acid-Sodium Citrate Solution?

A

3-14 days

43
Q

What is the Decalcification time of Trichloroacetic Acid?

A

4-8 days

44
Q

What is the Decalcification time of Perenyi’s Fluid?

A

2-7 days

45
Q

What is the Decalcification time of Formol-Nitric Acid?

A

1-3 days

46
Q

What is the Decalcification time of Aqueous Nitric Acid Solution 10%?

A

12 - 24 hours

47
Q

What is the Decalcification time of Phloroglucin-Nitric Acid?

A

12-24 hours

48
Q

What Clearing agent are used when the tissue is to be cleared directly from water, as in a frozen section?

A

Glycerin and gum syrup

49
Q

T or F. Prolonged exposure to most clearing agents causes the tissue to become brittle and therefore more difficult to cut.

A

True

HISTOPATH LEC (4 decks)

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