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MICROPARA LAB > M2 PART2 > Flashcards

M2 PART2 Flashcards

1
Q

Sum of all chemical reaction that occur within a living cell

A

METABOLISM

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2
Q

Metabolic activity of bacteria in the presence of what enzymes:

A

ENDOENZYMES
EXOENZYMES

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3
Q

Energy-producing reactions of bacteria:

A

OXIDATIVE PROCESS
FERMNETATION PROCESS

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4
Q

bacteria catalyzes within the cell

A

ENDOENZYME

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5
Q

bacteria catalyzes outside the cell

A

EXOENZYME

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6
Q

with oxygen
CO2 + H2O

A

OXIDATIVE PROCESS

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7
Q

acids, alcohols, gases
facultative aerobes

A

FERMENTATION PROCESS

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8
Q
  • The substrate formed from the metabolism of carbohydrate is either glucose or lactose (some people lack enzymes that breaks them down)
  • used by almost all bacteria

Glucose —> acid, alcohol, gas, organic molecule

A

CARBOHYDRATE FERMENTATION

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9
Q

CARBOHYDRATE FERMENTATION

The substrate formed from the metabolism of carbohydrate is either ____ or ____.

A

GLUCOSE
LACTOSE

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10
Q

CARBOHYDRATE FERMENTATION

source of energy with or without oxygen

A

ATP

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11
Q

Fermentation in Broth Medium CULTURE MEDIA USED

A

LACTOSE BROTH

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12
Q

Fermentation in Broth Medium | CHANGE IN COLOR

Inability to utilize carbohydrates

A

RED TO RED

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13
Q

Fermentation in Broth Medium | CHANGE IN COLOR

Turbidity indicates growth in the medium. Color unchanged indicates other possible sources of energy such as peptone

A

RED TO RED (TURBID/CLOUDY)

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14
Q

Fermentation in Broth Medium | CHANGE IN COLOR

Ability to use carbohydrates and produce organic acid

A

RED TO YELLOW

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15
Q

Fermentation in Broth Medium | CHANGE IN COLOR

Ability to use carbohydrate and produce organic acids and gas.

A

RED TO YELLOW WITH BUBBLE IN DURHAM TUBE

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16
Q

RESULTS

negative fermentation reaction

A

RED, unchanged

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17
Q

RESULTS

fermentation of the carbohydrate, or acid conditions

A

RED TO YELLOW

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18
Q

RESULTS

fermentation as well as gas formation, or acid + gas

A

RED TO YELLOW w bubble in Durham tube

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19
Q

Fermentation in Solid Medium CULTURE MEDIA USED

A

TRIPLE SUGAR IRON (TSI)
DEEP SLANT

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20
Q

Fermentation in Solid Medium | TSI

inidcates acid production

A

RED TO YELLOW

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21
Q

Fermentation in Solid Medium | TSI

indicates hydrogen sulfide gas

A

BLACK COLOR

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22
Q

Fermentation in Solid Medium | TSI

POSITIVE
(glucose fermentation)

A

YELLOW DEEP
RED SLANT

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23
Q

Fermentation in Solid Medium | TSI

POSITIVE
(indicates glucose, lactose & sucrose fermentation)

A

YELLOW DEEP & SLANT

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24
Q

Fermentation in Solid Medium | TSI

NEGATIVE
(indicates that no sugar was fermented)

A

RED

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25
Q

Carbohydrate Fermentation TEST

A

IMViC Test
* Indole Production Test
* Methyl Red Test
* Voges-Proskauer Test
* Citrate Utilization Test

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26
Q

Methyl Red Test CULTURE MEDIA

A

Methyl Red-Voges Proskauer Broth (MRVP)

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27
Q

METHYL RED TEST

glucose produces

A

ACID

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28
Q

METHYL RED TEST

ph indicator

A

methyl red

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29
Q
A
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31
Q

METHYL RED TEST

pH: RED

32
Q

METHYL RED TEST

pH: YELLOW

33
Q

Vogues-Proskauer Test CULTURE MEDIA

A

Methyl Red-Voges Proskauer Broth
(MRVP)

34
Q

Vogues-Proskauer Test

glucose produces

butanediol fermentation

A

acetoin (acetyl methyl carbinol)

35
Q

Vogues-Proskauer Test

how many drops:
Barritt’s A (alpha-naphthol) in ethanol

36
Q

Vogues-Proskauer Test

Barritt’s B (40% KOH)

37
Q

It is used to differentiate between organisms that produce large amounts of acid and organisms that only produce neutral content (acetoin)

38
Q

MRVP TEST

positive result

39
Q

MRVP TEST

negative result

40
Q

Citrate Utilization Test CULTURE MEDIA

A

Simmons Citrate Agar (SCA) Slants

41
Q

Citrate Utilization Test

indicator

A

bromothymol blue

42
Q

Citrate Utilization Test

alkaline pH

43
Q

Citrate Utilization Test

neutral pH

44
Q

Citrate Utilization Test

NEGATIVE RESULT

45
Q

Citrate Utilization Test

indicates that the bacterium inoculated utilized the citrate for energy production.

POSITIVE RESULT

46
Q

Indole Production Test CULTURE MEDIA

A

Sulfide Indole Motility (SIM)

47
Q

Indole Production Test

indole-positive
negative for sulfide production

A

purple layer sa taas

48
Q

Indole Production Test

sulfide production
indole production

49
Q

Indole Production Test

negative reaction

50
Q

The IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) set of reactions used to differentiate between ____ and ____

A

E. COLI
ENTEROBACTER AEROGENES

51
Q

Lysine Decarboxylase Test CULTURE MEDIA

A

Lysine Iron Agar (LIA) Slants

52
Q

Lysine Decarboxylase Test

lysine produces

A

cadaverine & CO2

53
Q

Lysine Decarboxylase Test

indicator

A

bromcresol purple

54
Q

Lysine Decarboxylase Test

alkaline pH

55
Q

Lysine Decarboxylase Test

acidic pH

56
Q

Lysine Decarboxylase Test

H2S production

57
Q

Phenylalanine Deamination

phenylalanine produces

A

phenylpyruvic acid

58
Q

Phenylalanine Deamination

test reagents contains

59
Q

Phenylalanine Deamination

acid is present

60
Q

Phenylalanine Deamination

negative

61
Q

Urease Test

urea produces

62
Q

a by product of protein metabolism

63
Q

Urease Test

production of ____ results in the increase in pH of the medium

64
Q

Urease Test

indicator

A

phenol red

65
Q

Urease Test

ph <6.8

NEGATIVE

A

orange yellow

66
Q

Urease Test

ph >8.1

POSITIVE

A

bright pinkish red

67
Q

Hydrogen peroxide is one of the by-products of oxidation-reduction, in the presence of oxygen.
* H2O2 ———> water and oxygen

A

CATALASE TEST

68
Q

CATALASE TEST

____ is one of the by-products of oxidation-reduction, in the presence of oxygen.

A

HYDROGEN PEROXIDE

69
Q

Protects bacteria from** toxic hydrogen peroxide (H2O2) accumulation**, which can occur during aerobic metabolism. If hydrogen peroxide accumulates, it becomes toxic to the organism

A

Catalase enzyme

70
Q

used to determine a bacterium’s ability to reduce nitrates

A

nitrate broth

71
Q

Nitrate Reduction Test

Nitrate reagent A

A

dimethyl-alpha-naphthylamine

72
Q

Nitrate Reduction Test

Nitrate reagent B

A

sulfanilic acid

73
Q

Nitrate Reduction Test

positive result

74
Q

Nitrate Reduction Test

if it does not turn red, what to be added in small pinch

MICROPARA LAB (3 decks)

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