[M] Lec 03.1: Labeled Immunoassay Study Guuide

Question 1

Patient antigen and labeled antigen react with reagent antibody in solution. Enzyme label is inactivated when reagent antigens binds to antibody

Answer 1

Homogenous EIA

Question 2

No physical separation step of bound and labeled antigens required

Answer 2

Homogenous EIA

Question 3

Patient antigen competes with labeled antigen for limited antibody binding sites. Final detected signal is AgAb

Answer 3

Competitive

Question 4

Excess solid phase ANTIGEN binds with px antibody and a second labeled antibody is added

Answer 4

Noncompetitive, indirect ELISA for antibody detection

Question 5

Excess solid phase ANTIBODY binds with px antibody and a second labeled antibody is added after a wash step

Answer 5

Capture or sandwich

Question 6

Reagent antigen is bound to an enzyme tag. Change in enzyme activity is observed as specific antigen-antibody interaction occurs in solutin

Answer 6

EMIT

Question 7

To detect patient antibody, reagent antigen is attached to a slideand incubated with the patient sample

Answer 7

Indirect immunofluorescence

Question 8

Fluorescent-labeled antigen competes with patient antigen for a limited number of soluble anti-body-binding sites

Answer 8

Fluorescence polatization

Question 9

Patientt sample is added to a test strip and migrates through the strip. Patient antigen complexes to antibody-labeled particles or competes with antigen-labeled particles

Answer 9

Rapid immunochromatographic

Question 10

Directly proportional type of immunoassay

Answer 10

Homogenous
Heterogenous, noncompetitive
Noncompetitive indirect ELISA
Capture or sandwich
EMIT

Question 11

Inverse ratio types of Immunochromatography

Answer 11

Heterogenous, competitive
Competitive
Fluoresence polarization