LO4 - Microscopes

Question 1

Describe the stages in the calibration of the eyepiece graticule.

Answer 1

Eyepiece graticule is placed in the eyepiece of the microscope.
Stage micrometer is placed on stage of microscope.
Appropriate objective lens selected.
Eyepiece graticule rotated to appropriate orientation.
Graticule and micrometer lined up so that a suitable distance on the micrometer corresponds with divisions.
Reading made from scale on graticule against dimension.

Question 2

Suggest three advantages of using a Scanning Electron Microscope to produce an image.

Answer 2

Increased depth of field (3D image).
Reveals surface topography (detail).
Greater resolution.