LO2 - Thin Layer Chromatography

Question 1

State two stationary phases in Thin Layer Chromatography.

Answer 1

Silica gel
Aluminium oxide (Alumina)
Cellulose

Question 2

Suggest a non-destructive method of visualising the spots on a Thin Layer Chromatography plate.

Answer 2

Under ultraviolet light

Fluorescence

Question 3

Discuss the advantages and disadvantages of both Paper Chromatography and Thin Layer Chromatography.

Answer 3

TLC gives a faster run.
TLC gives better separation and greater resolution of spots.
TLC plates are easier to manipulate.
TLC plates are more durable than paper.
Paper Chromatography is cheaper.
Neither gives positive identification.
Both used as qualitative, not quantitative.