LIPIDS BIOCHEMISTRY LAB Flashcards
are a diverse group of nonpolar molecules that include fats, oils, waxes, sterols, and phospholipids. They play essential roles in various biological processes, including energy storage, cell membrane structure, hormone production, and vitamin transport.
Lipids
is based on the principle of “like dissolves like.”
solubility test
The __ of fatty acids demonstrates their nonpolar nature and differential solubility in polar and nonpolar solvents.
solubility test
The___ of fatty acids is a fundamental technique in lipid biochemistry that helps determine the polarity of fatty acids
solubility test
Solubility Test Functions
Classifying Fatty Acids
Characterizing Lipid Mixtures
Understanding Lipid Properties
Distinguishing between saturated fatty acids (insoluble in water, soluble in hexane (a nonpolar)) and unsaturated fatty acids (insoluble in water, soluble in hexane).
Classifying Fatty Acids
Cottonseed oil addition: Using a pipette/dropper, put __ drops of cottonseed oil in each of the test tubes.
1-2
Unsaturated fatty acids can undergo oxidative degradation in the presence of heat. This test utilizes this property to detect the presence of unsaturated fatty acids.
ACROLEIN TEST
a volatile compound with a pungent odor.
acrolein
The __ is used to detect the presence of unsaturated fatty acids.
acrolein test
Applications (Acrolein Test)
Identifying Unsaturated Fatty Acids
Assessing Lipid Quality
Understanding Lipid Chemistry
Reagent in Acrolein Test
KHSO4 (Potassium Hydrogen Sulfate/ Potassium Bisulfate)
A __indicates the presence of unsaturated fatty acids due to the reduction of potassium permanganate by acrolein.
purple color change
The presence of a ___ confirms the formation of acrolein, suggesting the presence of unsaturated fatty acids.
pungent odor
The___ suggests the absence of unsaturated fatty acids and the minimal formation of acrolein.
absence of a color change
