LIPIDS BIOCHEMISTRY LAB Flashcards
are a diverse group of nonpolar molecules that include fats, oils, waxes, sterols, and phospholipids. They play essential roles in various biological processes, including energy storage, cell membrane structure, hormone production, and vitamin transport.
Lipids
is based on the principle of “like dissolves like.”
solubility test
The __ of fatty acids demonstrates their nonpolar nature and differential solubility in polar and nonpolar solvents.
solubility test
The___ of fatty acids is a fundamental technique in lipid biochemistry that helps determine the polarity of fatty acids
solubility test
Solubility Test Functions
Classifying Fatty Acids
Characterizing Lipid Mixtures
Understanding Lipid Properties
Distinguishing between saturated fatty acids (insoluble in water, soluble in hexane (a nonpolar)) and unsaturated fatty acids (insoluble in water, soluble in hexane).
Classifying Fatty Acids
Cottonseed oil addition: Using a pipette/dropper, put __ drops of cottonseed oil in each of the test tubes.
1-2
Unsaturated fatty acids can undergo oxidative degradation in the presence of heat. This test utilizes this property to detect the presence of unsaturated fatty acids.
ACROLEIN TEST
a volatile compound with a pungent odor.
acrolein
The __ is used to detect the presence of unsaturated fatty acids.
acrolein test
Applications (Acrolein Test)
Identifying Unsaturated Fatty Acids
Assessing Lipid Quality
Understanding Lipid Chemistry
Reagent in Acrolein Test
KHSO4 (Potassium Hydrogen Sulfate/ Potassium Bisulfate)
A __indicates the presence of unsaturated fatty acids due to the reduction of potassium permanganate by acrolein.
purple color change
The presence of a ___ confirms the formation of acrolein, suggesting the presence of unsaturated fatty acids.
pungent odor
The___ suggests the absence of unsaturated fatty acids and the minimal formation of acrolein.
absence of a color change
is a deterioration process that occurs in fats and oils due to oxidation. This oxidation leads to the formation of various compounds, including hydroperoxides, aldehydes, and ketones, which contribute to unpleasant odors and flavors and can also produce harmful compounds.
Rancidity
Assessing rancidity is crucial for:
Evaluating Food Quality
Monitoring Lipid Storage
Understanding Lipid Oxidation
Sterols, such as cholesterol, react with a mixture of ___ to produce a characteristic red-violet color. This color change is due to the formation of a conjugated diene system in the sterol molecule.
acetic anhydride and concentrated sulfuric acid
The____ is used to detect the presence of sterols, such as cholesterol. It involves a color reaction between sterols and a mixture of acetic anhydride and concentrated sulfuric acid.
Liebermann-Burchard test
Reagents in Liebermann-Burchard test
acetic anhydride and concentrated sulfuric acid.
A __ indicates the presence of sterols.
red-violet color change
___is a process in which a fat or oil is dispersed into small droplets in an aqueous medium. This process is essential for fat digestion and absorption in the body.
Emulsification
Emulsifying agents, such as ___, help to stabilize the emulsion by reducing the interfacial tension between the oil and water phases.
egg yolk or lecithin
___provide valuable tools for understanding the structure, properties, and functions of lipids. These techniques are essential for research in areas such as nutrition, metabolism, and human health
Lipid biochemistry techniques
