lipids 2

Question 1

it measures the total cholesterol in serum or plasma

Answer 1

enzymatic method

Question 2

color development mixture liebermann burchardt reagent

Answer 2

glacial acetic acid
acetic anhydride
concentrated H2SO4

Question 3

interferences in enzymatic measurement of total cholesterol

Answer 3

ascorbic acid
hemoglobin
bilirubin
plant sterols

Question 4

It uses hexane extraction after hydrolysis with alcoholic KOH followed by reaction with Liebermann-Burchardt color reagent.

Answer 4

CDC Reference Method (Abell, Levy, and Brodie Method)

Question 5

CDC Reference Method (Abell, Levy, and Brodie Method) end color

Answer 5

green

Question 6

It is the highest order method for serum cholesterol developed by NIST

Answer 6

isotope dilution mass spectrometry

Question 7

the gold standard method for cholesterol

Answer 7

isotope dilution mass spectrometry

Question 8

isotope dilution mass spectrometry is developed by

Answer 8

NATIONAL INSTITUTE OF STANDARD AND TECHNOLOGY

Question 9

NEUTRAL FAT

Answer 9

TRIGLYCERIDES/
TRIACYLGLYCEROL

Question 10

t contains 3 molecules of fatty acid and one molecule of gLycerol by

Answer 10

TRIGLYCERIDES
ESTROGEN BOND

Question 11

It is the main storage lipid in man (adipose tissue) - constitutes 95% of stored fat and the predominat form of glyceryl ester found in plasma.

Answer 11

TAG

Question 12

fasting req of TAG

Answer 12

10-12 hrs

Question 13

ref value TAG

Answer 13

< 150mg/dl

Question 14

borderline high TAG

Answer 14

150-199 mg/dl

Question 15

high tag

Answer 15

200-499 mg/dl

Question 16

very high TAG

Answer 16

> 500 mg/dl

Question 17

interference of TAG

Answer 17

ascorbic acid
bilirubin
hemolysis

Question 18

TAG level increases to ___ betwen ___ yrs old

Answer 18

2 mg/dl/year
45-65

Question 19

specific, rapid and easy to perform

Answer 19

enzymatic method

Question 20

major interference of enzymatic method

Answer 20

glycerol

Question 21

It involves hydrolysis of triglycerides to free fatty acids and glycerol, followed by the phosphorylation of glycerol to glycerophosphate.

Answer 21

Glycerol Kinase Method

Question 22

disappearance of NADH is measured at

Answer 22

340 nm

Question 23

Is a time-consuming manual method which cannot be automated.

Answer 23

modified van handel and zilversmith

Question 24

modified van handel and zilversmith end color

Answer 24

pink

Question 25

It involves alkaline hydrolysis (saponification) using alcoholic KOH, solvent extraction with chloroform

Answer 25

modified van handel and zilversmith

Question 26

The method measures total glycerides, which is defined as the sum of tri-, di, and monoglycerides plus any free glycerol.

Answer 26

GC-IDMS

Question 27

THE MOST HYDROPHOBIC LIPIDS

Answer 27

CHOLESTEROL ESTERS AND TRIGLYCERIDES

Question 28

In the endogenous pathway, production of TAG from FA by the liver take place, with synthesis of VLDL particles containing ____

Answer 28

apo B100 and apo E.

Question 29

considered to be an alternative pathway for HDL metabolism.

Answer 29

Cholesterol ester transfer protein

Question 30

hydrolyze TAG and cholesterol ester

Answer 30

lipoprotein lipase

Question 31

hydrolyze TAG and phospholipids from HDL

Answer 31

hepatic lipase

Question 32

catalyzes the esterification of cholesterol from HDL; enables HDL to accumulate cholesterol as cholesterol ester.

Answer 32

Lecithin Cholestero! Acyl Transferase (LCAT) -

Question 33

hydrolyzes phospholipids and TAG in HDL.

Answer 33

Endothelial lipase -

Question 34

for efflux of cholesterol from peripheral cells into HDL

Answer 34

ATP-binding cassette protein A1 (ABCA1) -

Question 35

These are large macromolecular complexes of lipids

Answer 35

lipoproteins

Question 36

specialized proteins known as

Answer 36

apolipoproteins.

Question 37

To transport TAG and cholesterol to sites of energy storage and utilization

Answer 37

lipoproteins

Question 38

a fat-soluble vitamin) depends upon chylomicrons for absorption and relies upon VLDL and LDL for delivery to tissues.

Answer 38

vit. e

Question 39

It interacts with specific cell surface receptors and directs the lipids to the correct target organs and tissues in the body; present on the surface of lipoprotein particles.

Answer 39

apolipoprotein

Question 40

Major apolipoproteins:

Answer 40

ApoA-1, ApoB-48, and ApoB-100

Question 41

It is the largest and least dense of the lipoprotein particles.

Answer 41

chylomicrons

Question 42

major apolipo of chylomicrons

Answer 42

apoB-48

Question 43

density of chylomicrons

Answer 43

<0.95 kg/l

Question 44

it is secreted in the liver.* It transports endogenous TAG from the liver to muscle, fat depots, and peripheral tissues.

Answer 44

Pre-Beta Lipoprotein/Very-Low-Density Lipoprotein (VLDL)

Question 45

major apolipo of VLDL

Answer 45

apoB-100

Question 46

It is the smallest lipoproteins but the most dense (5-12 nm). It is produced in the liver and intestine (nascent disk-shaped particles).

Answer 46

Alpha Lipoprotein /High Density Lipoprotein (HDL)

Question 47

It is synthesized in the liver. It is the major end product from the catabolism of VLDL. MAJOR APOLIPO

Answer 47

Beta Lipoprotein/Low-Density Lipoprotein (LDL) APO B-100

Question 48

It is a product of VLDL catabolism - "VLDL remnant."

Answer 48

Intermediate Density Lipoprotein (IDL) APOB-100

Question 49

It is similar to LDL (density and composition

Answer 49

LIPOPROTEIN (a) apo (a)

Question 50

It is an abnormal lipoprotein found in obstructive jaundice and LCAT deficiency. it is a specific and sensitive indicator of cholestasis.

Answer 50

lipoprotein x apoB-100 and apo (a)