Lesson 2.6 Flashcards
1
Q
Biotechnology:
A
Cloning and GMOs
2
Q
cloning:
A
for a gene –> one protein -whole organism expensive
3
Q
GMO:
A
genetically modified organism
4
Q
DNA sequencing :
A
order and identity
5 to 3 every base
5
Q
Locating specific genes:
A
mRNA –> cDNA(copied) –>sequence this
- read from it
- looking for open frames(proteins)
6
Q
Restriction endonucleases:
A
scissors: enzymes (proteins) that cleaves DNA
7
Q
Ligase:
A
makes phosphodiester bodies
“tape or paste”
reforms DNA backbone
8
Q
Vectors:
A
Delivery system
9
Q
Gel Electrophoresis:
A
method for separation and observing the results of manipulation
DNA or RNA move toward + electrode since backbone in negatively charged
10
Q
Polymerase Chain Reaction (PCR)
A
amplifying or copying DNA
