lesson 12

Question 1

central dogma

Answer 1

1. DNA
   Transcription (RNA synthesis)
2. RNA
   Translation (protein synthesis)
3. Protein

Question 1

eukaryotic transcription

Answer 1

occurs in the nucleus
three different RNA polymerases
-RNA polymerase ll trancribes mRNA
promoter position and sequence differs from prokaryotes
a series of transcription factors recrui and activate RNA pol 2
termination sites ar enot well defines
primary transcripts are processed to produce mature mRNA

Question 2

translation

Answer 2

process of protien synthesis(converts RNA sequence into polypeptide sequence)
basics same in prokaryotes and ukaryotes

Question 3

initiation: recognition of translation start site (p

Answer 3

1. small subunit binds RBS on mRNA to determine translation start site
2. initator tRNA N formyl met bonds to start codon in the p site
3. large subunit binds
4. a site open to accept next trna-aa
5. switch to elongation

Question 4

initation: reconition of translation start (eukaroytes

Answer 4

1. small subunit binds RBS on mRNA to determine translation start site
2. initiator tRNA N formyl met bonds to start codon in the p site
3. large subunit binds
4. a site open to accept next trna-aa
5. switch to elongation

Question 5

termination of translation

Answer 5

elongation continues until stop codon (UAA, AG, UGA) is reached
triggers realse of a new polypeptide from P-site tRNA