Lentivirus

Question 1

Type of vector used

Answer 1

Replication‐incompetent vector—and if it’s further engineered with mutations that disable integration, it would be non‐integrative as well.

Question 2

Safety of the vectors

Answer 2

1)It has mutations in the genome for not be able to integrate the DNA (the integrase D64V mutation in Pol gene)
2) Multiple plasmids, no single plasmid contains all the viral genetic information, there’s no way for the full set of viral genes to be reassembled in the infected cell.
3) Incorporates a deletion in the U3 region of the 3′ long terminal repeat (LTR). This “self-inactivating” (SIN) design prevents the LTR from driving transcription after integration

Question 3

Which gene carries the reporter gene?

Answer 3

Lentiviral “backbone”

Question 4

In which gene you can find the integrase of a lentivirus?

Answer 4

Integrase enzyme is encoded by the pol (polymerase) gene