Lecture9 Flashcards
MTS - is?
Mitochondrial targeting signal
for an approach to target specific mutations, we can use FOKL which is a
nuclease
What are the Challenges associated with live-cell microscopy?
• Bleaching
(fading of the fluorescent protein)
• Phototoxicity
(too much light is toxic for cells)
What is the Mitochondrial Fusion?
2 Mitochondria fuse to form 1 mitochondrion
What is the Mitochondrial Fission?
1 Mitochondrion divides into 2 daughter mitochondria
mgm1 is highly conserved in mammals called…
opa1
What does Nebenkern mean?
Aggregated mitochondria that appear close to the nucleus during spermatogenesis
FZO (Fuzzy onion) is Discovered in…
Drosophila
S. cerevisiae—> FZO1
Mammals —>
MFN1/MFN2
Mitofusins
mgm1 is localized in …
FZo1 localized in …
IM and a big part of it is exposed into IMS, FZO1 OM
What do you know about DNM1?
- Isolated in a visual yeast screen
* First component of the mitochondrial fission machinery
to which family do DNM1, FZO1, and mgm1 belong?
Dynamin like proteins
For fussion we have:
Fzo1 / Mfn1, Mfn2
For fission we have :
Dnm1/ Drp1
What its cycloheximide function?
is translation inhibitor and it inhibit translation in cytosol
Mfn1/2 can dimerize in trans via their cytoplasmic… domains
coiledcoil
When the GTPase domain of Mitofusins is mutated, in Which stage mitochondrial fusion is trapped?
at the tethering stage
Mutation of the GTPase domain in Mitofusin results in
aggregation of mitochondria, mitochondria are tethered and a
gap can be observed between mitochondria
What do you think?
yes
Mgm1/Opa1 are imported for fusion of…
IM
… and … are required for mitochondrial fusion
GTP and Membrane potential
Fzo1 =
Mitofusins
Purified Dnm1/Drp1 can form oligomeric rings.
True or false
True
High mitochondrial activity leads to high….
ROS
Neurons have a high energy demand, especially at…
synapses
Neurons crucially depend on mitochondrial transport
yes
…. interacts with Kinesin
Milton interacts with Kinesin
Neither Milton nor KHC has a mitochondrial targeting signal or a
transmembrane segment
True
Milton interacts with…, a mitochondrial protein
Miro, a mitochondrial protein
Ca2+ inhibits mitochondrial motility
yes
Calcimycin leads to a rise in intracellular Ca2+ content
true
