Lecture5

Question 1

Precursors proteins are bound by small TIM chaperones in the IMS (Tim9, 10, 12) and then Tim22 mediates insertion into the inner membrane in a ΔΨ independent manner
False or True?

Answer 1

False, Tim22 mediates insertion into the inner

the membrane in a ΔΨ dependent manner

Question 2

Erv1 (contains FAM) right?

Answer 2

no, Erv1 (contains FAD)

Question 3

Tim22 to IM and Import of beta-barrel proteins The substrate is bound by the small Tim chaperones
T or F?

Answer 3

True

Question 4

The small Tim chaperones shuttle proteins to either the SAM or the TIM22 complex
False or True?

Answer 4

True

Question 5

The Tim22 complex mediates import of…

Answer 5

mitochondrial carrier proteins

Question 6

Recently described rescue responses are?

Answer 6

-mPOS
mitochondrial precursor overaccumulation stress
-mitoCPR
mitochondrial compromised protein import response
-UPRam
unfolded protein response activated by mistargeted proteins
-mitoTAD
mitochondrial protein translocation-associated
degradation

Question 7

Sod2, Rip1 and Mdj1 are three
proteins with cleavable presequences
True or False?

Answer 7

True

Question 8

What are the roles of Cardiolipin?

Answer 8

Specific to mitochondria Roles in:
• Apoptosis
• Stability of supercomplexes
• Mitochondrial fusion
• Mitochondrial import

Question 9

Cardiolipin is present virtually only in …. and its highly enriched in the…

Answer 9

mitochondira, IM